Lonza Inc.

Robert Young

Peter Michael O'callaghan

Colin Mark Jaques

Stephen Jaffe

James Budge

Joanne Robool

Andrew J Racher

Thomas Payne

Christopher Mark Smales

Gurdeep Singh

David James

Collin Mark Jaques

The present disclosure features methods, cells or cell lines, and compositions for increasing the amount of products, e.g., proteins, produced by a cell or cell line by reducing the level of a non-essential endogenous protein.

Methods for improving production of biological products by reducing the level of endogenous protein

Described herein are compositions and methods for identifying, selecting, or culturing cells comprising a subject nucleic acid sequence of interest. Generally, a nucleic acid comprising a subject nucleic acid and a sequence encoding an enzyme molecule involved in biosynthesis of an amino acid is introduced into a cell. The cell is then grown on media lacking the amino acid, such that cells comprising the introduced nucleic acid are capable of growth. In some instances, the cell further comprises an inhibitor of the enzyme molecule to increase the stringency of the selection.

Methods of cell selection and modifying cell metabolism

Disclosed are genetic control circuits, cells, and methods that use a repressor polypeptide to reduce the transcription rate of an exogenous therapeutic polypeptide encoding gene in response to a change in condition.

Universal self-regulating mammalian cell line platform for the production of biologics

Growing community of inventors

Visp, Switzerland

Robert Young