Sensors for Medicine & Science, Inc.

Life Technologies Corporation

Senseonics, Incorporated

Robert William Lynn

Arthur Earl Colvin

Paul Samuel Zerwekh

Casey J O'Connor

Carrie R Lorenz

Steven J Walters

Jeffrey C Lesho

Robert W Flesher

Robert W Blakesley

Kevin J Barnes

David J Cady

Kevin R Hall

Casey J O'connor

Gregory A Dale

Jeffery C Lesho

Kevin T Barnes

Paul S Zerwekh

The present invention provides systems and methods for attenuating the effect of ambient light on optical sensors and for measuring and compensating quantitatively for the ambient light.

System and method for attenuating the effect of ambient light on an optical sensor

An electro-optical sensing device for detecting the presence and concentration of an analyte in a liquid or gaseous medium includes a pair of indicator elements positioned to receive radiation from a radiation source and transmit radiation to a pair of photosensitive elements. The indicator elements each contain indicator molecules having an optical characteristic responsive to the presence of an analyte; however, one of the indicator elements is covered by an analyte-impermeable chamber that renders the indicator element insensitive to the presence of the analyte in the medium outside the chamber so that it can be used as a reference to cancel environmental and systemic variables that affect both indicator elements. The chamber preferably holds an analyte-containing fluid in contact with the reference indicator element so that the indicator elements operate under nominally identical conditions. The indicator element used to measure the analyte in the external medium is preferably also covered, but in a manner that provides direct contact between the analyte and the indicator element.

Electro-optical sensing device with reference channel

An optical-based sensor for detecting the presence or amount of an analyte using both indicator and reference channels. The sensor has a sensor body with a source of radiation embedded therein. Radiation emitted by the source interacts with indicator membrane indicator molecules proximate the surface of the body. At least one optical characteristic of these indicator molecules varies with analyte concentration. For example, the level of fluorescence of fluorescent indicator molecules or the amount of light absorbed by light-absorbing indicator molecules can vary as a function of analyte concentration. In addition, radiation emitted by the source also interacts with reference membrane indicator molecules proximate the surface of the body. Radiation (e.g., light) emitted or reflected by these indicator molecules enters and is internally reflected in the sensor body. Photosensitive elements within the sensor body generate both indicator channel and reference channel signals to provide an accurate indication of the concentration of the analyte. Preferred embodiments are totally self-contained and are sized and shaped for use in vivo in a human being. Such embodiments preferably include a power source, e.g. an inductor, which powers the source of radiation using external means, as well as a transmitter, e.g. an inductor, to transmit to external pickup means the signal representing the level of analyte.

Optical-based sensing devices

Disclosed is a system for dispensing and supplementing a large volume of liquid for use with cell and tissue cultures. The system disclosed comprises a large volume drum having liquid contained therein. The system disclosed further comprises a first assembly for dispensing the liquid from the large volume drum. The system disclosed further comprises a second assembly for supplementing the first liquid contained in the large volume drum with the second liquid. The system disclosed may also comprise a third mechanical arrangement for mixing the first and second liquids.

Large scale liquid media dispensing and supplementing system

Disclosed is a system for dispensing and supplementing a large volume of liquid for use with cell and tissue cultures. The system disclosed comprises a large volume drum having liquid contained therein. The system disclosed further comprises a first assembly for dispensing the liquid from the large volume drum. The system disclosed further comprises a second assembly for supplementing the first liquid contained in the large volume drum with the second liquid. The system disclosed may also comprise a third mechanical arrangement for mixing the first and second liquids. The materials chosen are compatible with gamma irradiation sterilization process, sterile cell culture media, and standard distribution systems.

Electrophoresis sequencing apparatus

Electrophoresis sequencing apparatus tray

A vertical gel slab electrophoresis apparatus is disclosed comprised of a vertically oriented gel slab arranged between upper and lower buffer solution reservoirs. A bifurcated movable lower tray contains both an upper buffer reservoir drain and lower buffer reservoir. Both upper and lower buffer reservoirs are provided with hinged covers which act as electrical interlocks to prevent unsafe operation of the apparatus, as well as prevent spillage of buffer solution. The design overcomes safety problems associated with both electrical shock and radioactive chemical contamination. The apparatus is provided with handles for each relocation. Cooling vents are provided for ambient air cooling of the apparatus. A gel mold experiment can be assembled and disassembled with unusual ease with the aid of unique self-locking clamp assemblies.

Vertical gel slab electrophoresis apparatus

Growing community of inventors

Germantown, MD, United States of America

Robert William Lynn