Forschungszentrum Juelich Gmbh

Forschungszentrum Julich Gmbh

Robert Westphal

Martina Pohl

Doerte Rother

Torsten Sehl

Lisa Marx

Dörte Rother

A lyase has an amino acid sequence selected from SEQ ID NOs: 1, 2 and 3, wherein the amino acid isoleucine in position no. 468 in the protein ApPDC-E469G, which is modified with respect to the wild type from, is replaced by an amino acid which occupies less space than isoleucine.

Lyase and method for asymmetric synthesis of (S)-phenylacetylcarbinol

The invention relates to a lyase and a lyase-encoding DNA, to vectors containing the DNA and to a method for the asymmetric synthesis of (S)-phenylacetylcarbinol. According to the invention, a lyase is provided, in which tryptophan is replaced with an amino acid at position 543 in protein ApPDC-E469G, said protein being modified with respect to the wild type of, or in which it is less space-filling than tryptophan. According to the invention, deoxyribonucleic acids are furthermore provided, which encode the lyase. (S)-phenylacetylcarbinol can be produced with the lyase according to the invention from the educts benzaldehyde and pyruvate or acetaldehyde with an enantiomeric excess of at least 94%.

Lyase and lyase-encoding DNA, vectors containing the DNA, and method for the asymmetric synthesis of (S)-phenylacetylcarbinol

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Leipzig, Germany

Robert Westphal