Third Wave Technologies, Inc.

Northwestern University

Qiagen North American Holdings, Inc

Robert W Kwiatkowski, Jr

Victor I Lyamichev

Jeff G Hall

Bruce P Neri

Andrea L Mast

Hon Ip

Christian Tor Bartholomay

Hatim T Allawi

Peggy S Eis

Michael W Kaiser

Marilyn C Olson-Munoz

Sarah Olson

Andrew A Lukowiak

LuAnne Chehak

Michelle Curtis

Tsetska Y Takova

James J Schaefer

Kevin L Vedvik

WuPo Ma

Mary Ann D Brow

Lin Ji

Zbigniev Skrzypczynski

Natalie E Lyamicheva

Brad T Argue

Lloyd M Smith

David M Kelso

Poonam Agarwal

Lisa C Thompson

Stephanie H Vavra

Priscilla Wilkins Stevens

Kim Paulsen

John M Wages

Angela Hudson

Robert Jackson Bair

Michelle Garsha

Patrick Peterson

Molly Accola

Susan S Wigdal

Kristen Campbell Benedict

Zbigniew Skrzypczynski

Natalie Lyamichev

Erin Dorn

Vincent Tevere

Daniel A Strom

Nancy Jarvis

Wendy J Kivens

Slava Elagin

Wendy J Kivins

Hon S Ip

Kathleen Carroll

Laura Heisler

Molly A Accola

The present disclosure provides methods for isolating substantially pure and undegraded DNA from biological material via a solid support. The methods may use a DNA lysing solution that comprises a surfactant, a DNA-complexing salt (e.g., a lithium salt), and a buffer. Alternatively, the methods may use a solid support pre-treated with the DNA lysing solution.

Compositions and methods for using a solid support to purify DNA

The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5' nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.

RNA detection assays

The present invention provides compositions and methods for the detection and characterization of mutations associated with non-syndromic hearing impairment. More particularly, the present invention provides compositions, methods and kits for using invasive cleavage structure assays (e.g. the INVADER assay) to screen nucleic acid samples, e.g., from patients, for the presence of any one of a collection of mutations in the Connexin 26, or gap junction beta 2, gene associated with non-syndromic hearing loss.

Connexin allele detection assays

The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of nucleic acid from various viruses in a sample.

Kits for detection of nucleic acids using invasive cleavage structures and flap endonucleases

The present invention relates to systems, compositions, and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for attaching nucleic acids to solid supports and modifying nucleic acids. For example, in some embodiments, the 5' nuclease activity of a cleavage agent is used to cleave a cleavage structure formed on the solid support, the occurrence of the cleavage event indicating the presence of specific nucleic acid sequences.

Solid-phase reactions

Kits for detection of nucleic acids by invasive cleavage

The present invention provides compositions and methods for the detection and characterization of mutations associated with cystic fibrosis. More particularly, the present invention provides compositions, methods and kits for using invasive cleavage structure assays (e.g. the INVADER assay) to screen nucleic acid samples, e.g., from patients, for the presence of any one of a collection of mutations in the CFTR gene associated with cystic fibrosis. The present invention also provides compositions, methods and kits for screening sets of CFTR alleles in a single reaction container.

CFTR allele detection assays

Detection of RNA Sequences

Nucleic acid detection kits

Growing community of inventors

Verona, WI, United States of America

Robert W Kwiatkowski, Jr