Bio-rad Laboratories, Inc.

Robert V Dahlstrom

James F Monthony

Michael W Burgett

Charles F Shannon

An assay method suitable for antigens (or haptens) including proteins and polypeptides utilizing antibodies for the unknown protein or polypeptide in which the antibodies are covalently bound to water insoluble hydrophilic polymeric particles. Appropriate fluorescently labeled immune reactants are introduced during the method and together with the unknown antigen are immunologically bound, directly or indirectly, to the particles for separation from the reaction liquid. The particles have a size of about 0.1-10 microns and can be resuspended for direct measurement of the fluorescent labels in a fluorometer from which the unknown can be determined.

Reagent combination for solid phase immunofluorescent assay

An assay method suitable for antigens (or haptens) including proteins and polypeptides utilizing antibodies for the unknown protein or polypeptide in which the antibodies are covalently bound to water insoluble hydrophylic polymeric particles. Appropriate fluorescently labeled immune reactants are introduced during the method and together with the unknown antigen are immunologically bound, directly or indirectly, to the particles for separation from the reaction liquid. The particles have a size of about 0.1-10 microns and can be resuspended for direct measurement of the fluorescent labels in a fluorometer from which the unknown can be determined.

Solid phase immunofluorescent assay method

A method for determining serum thyroxine (T-4) in which the T-4 is first separated from the serum by adsorption onto a montmorillonite clay and then competitively bound by exogenous thyroxine binding globulin in the presence of a known amount of radioactive T-4. The competitively bound serum T-4 and radioactive T-4 is separated from the unbound serum T-4 and radioactive T-4 by passage through an ion exchange resin column. The serum T-4 concentration is determined by measuring the amount of bound radioactive T-4 and referring to a standard curve.

Growing community of inventors

San Rafael, CA, United States of America

Robert V Dahlstrom