Life Technologies Corporation

Robert Tebbs

Olga Petrauskene

Pius Brzoska

Craig Cummings

Priya Balachandran

Paolo Vatta

Kelly Li

Lily Wong

Ioanna Pagani

Kamini Varma

Michael Tanner

Hong Ji

Jisheng Li

Patrick Zoder

Chunling Wang

Fangqi Hu

Kathleen Hayashibara

Manohar R Furtado

Jen-Kuei Liu

Arlene Nunez

Payman Fatemi

Somaya Bit

Compositions, assays, methods, diagnostic methods, kits, and diagnostic kits are disclosed for the specific and differential detection of SARS-CoV-2 and/or other viruses from samples, including veterinary samples, clinical samples, food samples, forensic sample, environmental samples (e.g., obtained from soil, garbage, sewage, air, water, food processing and manufacturing surfaces, or likewise), or biological sample obtained from a human or non-human animal.

Compositions, kits and methods for detection of viral sequences

Disclosed is a multi-primer amplification assay, method and kits for detectingspecies and closely related species utilizing a plurality of oligonucleotide primers in contact with a sample in a single vessel and detecting the amplification product, wherein the presence of an amplification product indicatesin the sample.

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Embodiments of the disclosure relate to isolated nucleic acid sequences, methods of use thereof, and workflows for detection of severalspecies in a sample, particularly in a food or environmental sample. Embodiments of the disclosure may also be used to detect one or more species or strains offrom each other, for examplemay be detected independently of otherspp. Some embodiments also describe a duplexed assay that can detect(formerly-),, and. Kits for detection ofare also described. In some embodiments, methods and kits of the disclosure may comprise a TAQMAN&#xae; assay. In some embodiments, 0.2-2 cfu ofspp. are detected using the compositions, methods and kits after a 24-28 hour enrichment period.

Embodiments of the disclosure relate to compositions of isolated nucleic acid sequences, methods, workflows and kits of use thereof for detection of, subspecies, serovar() in a sample, particularly in a food (egg, poultry) or environmental sample (including poultry-related environmental samples). Embodiments of the disclosure may also be used to differentially detectwithout cross-reacting withC or other closely relatedspecies. In some embodiments, methods and kits of the disclosure may comprise a TaqMan&#xae; assay. Following sample enrichment, methods of detection may be completed in approximately 3 hours.

Embodiments of the disclosure relate to isolated nucleic acid sequences, methods of use thereof, and workflows for detection of severalspecies in a sample, particularly in a food or environmental sample. Embodiments of the disclosure may also be used to detect one or more species or strains offrom each other, for examplemay be detected independently of otherspp. Some embodiments also describe a duplexed assay that can detect(formerly incertae-sedis),, and. Kits for detection ofare also described. In some embodiments, methods and kits of the disclosure may comprise a TAQMAN&#xae; assay. In some embodiments, 0.2-2 cfu ofspp. are detected using the compositions, methods and kits after a 24-28 hour enrichment period.

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Austin, TX, United States of America

Robert Tebbs