Roche Diagnostics Gmbh

Immunomedics, Inc.

Roche Diagnostic Systems, Inc.

Roche Diagnostics Operations, Inc.

Robert Sundoro Wu

Salvatore Joseph Salamone

Hans J Hansen

Mitali Ghoshal

Stephen S Vitone

Dan Shochat

Min Li

Jane S C Tsai

Kathryn Sarah Schwenzer

A water-soluble reference standard is useful for immunoassays of a lipophilic drugs. The reference standard is a compound of formula (I):G-(L)&#x2014;Y;&#x2003;&#x2003;(I)where G is a lipophilic drug; L is a linker which is an alkyl group or heteroalkyl group containing from 1 to 20 carbon atoms; n is 0 or 1; and Y is a water-solubilizing group such as &#x2014;SO, &#x2014;NR&#x2014;SO, &#x2014;P(&#x2550;O)(OH)(O), or &#x2014;O&#x2014;P(&#x2550;O)(OH)(O); where R is H or an alkyl group of 1 to 10 carbon atoms.

Water-soluble derivatives of lipophilic drugs

The present invention provides hapten derivatives that are useful for the preparation of antigens, antibodies and reagents having superior performance characteristics for use in immunoassays for the detection of LSD and nor-LSD. In the present invention the LSD nucleus is derivatized out of the indole nitrogen to form an aminoalkyl derivative. Derivatives have also been synthesized out of the piperidine nitrogen of the LSD nucleus. The resulting haptens can then be further modified at these functionalized positions for linking to appropriate antigenic or labelling groups to provide reagents for LSD immunoassays having excellent sensitivity and selectivity for both LSD and nor-LSD.

Reagents for lysergic acid diethylamide immunoassay

The present invention provides hapten derivatives that are useful for the preparation of antigenic, antibody and label reagents having superior performance characteristics for use in immunoassays for the detection of d-propoxyphene and d-nor-propoxyphene. In the present invention the propoxyphene nucleus is derivatized out of the nitrogen center to form an aminoalkyl -carboxyl, or -hydroxyl haptenic derivative. The resulting hapten can then be further modified at the now functionalized position off the nitrogen for linking to an appropriate antigenic or labeling group to provide reagents for propoxyphene immunoassays having excellent sensitivity and selectivity for both d-propoxyphene and d-nor-propoxyphene.

Propoxyphene derivatives for immunoassay reagents

The present invention relates to a novel class of tracer compounds for fluorescence polarization imunoassays. The novel 4'-methyl fluorescein derivatives are conjugated to ligands via cyclic linkers.

4'-methyl substituted fluorescein derivatives

The present invention provides hapten derivatives that are useful for the preparation of antigenic, antibody and label reagents having superior performance characteristics for use in immunoassays for the detection of d-propoxyphene and d-nor-propoxyphene. In the present invention the propoxyphene nucleus is derivatized out of the nitrogen center to form an aminoalkyl -carboxyl, -amino, -thiol or -hydroxyl haptenic derivative. The resulting hapten can then be further modified at the now functionalized position off the nitrogen for linking to an appropriate antigenic or labelling group to provide reagents for propoxyphene immunoassays having excellent sensitivity and selectivity for both d-propoxyphene and d-nor-propoxyphene.

Methods of making propoxphene derivatives

Novel quinidine derivatives are provided which can be used in an improved immunoasssay for the detection of quinidine and quinidine metabolites.

Quinidine immunoassay and reagents

A protein containing at least one pendant sulfhydryl group is directly radiolabeled with a radiometal which binds tightly to sulfhydryl groups, using one or more pendant sulfhydryl groups on the protein as endogenous ligands and optionally using an exogenous ligand which binds tightly to the radiometal ion to further stabilize the chelate.

Method for radiolabeling antibody fragments

A protein containing at least one pendant sulfhydryl group is directly radiolabeled with a radiometal which binds tightly to sulfhydryl groups, using one or more pendant sulfhydral groups on the protein as endogenous ligands and optionally using an exogenous ligand which binds tightly to the radiometal ion to further stabilize the chelate.

Growing community of inventors

West Orange, NJ, United States of America

Robert Sundoro Wu