University of California

Medimmune Limited

Medlmmune Limited

Research Institute at Nationwide Children's Hospital

Robert Strouse

Christopher K Breuer

Alan Hunter

Hyongsok Tom Soh

Timothy Pabst

Xiangyang Wang

Narutoshi Hibino

Michaela Wendeler

Jihong Wang

Cameron Best

Yong Ung-Lee

Andrew Csordas

Faye Walker

James B McGivney Iv

Kendall Carey

Johnson Varghese

James B Mcgiveney Iv

This application provides a method of using aptamers to determine the presence of HCP's in a recombinant protein preparation, and provides methods of preparing a plurality of aptamers for use in the method of detecting a plurality of HCP's in a recombinant protein preparation. In accordance with the description, a method of determining the presence or absence of a plurality of HCP's in a recombinant protein preparation comprises: providing a recombinant protein preparation; providing a final pool of aptamers; combining the recombinant protein preparation with the final pool of aptamers; and determining the presence or absence of a plurality of HCP's.

Detecting residual host cell proteins in recombinant protein preparations

The present application relates to methods for purifying recombinant coagulation factor proteins, including for example, prothrombin (Factor II). In embodiments, the methods provide purified prothrombin that exhibit increased bioactivity and reduced levels of thrombin, thereby increasing the safety of the prothrombin. Also provided are purified recombinant coagulation factor proteins.

Methods for processing coagulation factors

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Gaithersburg, MD, United States of America

Robert Strouse