Applied Biosystems, LLC

Life Technologies Corporation

Ambion, Inc.

Robert Setterquist

Gary J Latham

Wade Lukianow

Timothy Barta

Ethan Gentert

Sara Thiele

Jay Greco

Liangjing Chen

Syrus Jaffe

Alexandre Vlassov

Robert Smith

Eric Liu

Justin Inslee

Jason Jones

Christopher Crowley

Carolyn Johnson

John Staton

Christopher Martin

Sandro David Klein

Richard Conrad

Brittan L Pasloske

Marie Gonzalez

Xiaohui Wang

Jon Kemppainen

Xingwang Fang

Xiaowei Wang

Robert Edwin Schneider

Andrew D Ellington

George L Murphy

Carolyn Adams

Allan Evans

Andrew Basta

Anne Cox

Mark Mayernick

Brian Vajda

Sumonto Ghosh

David Cerrone

Kraig Kruger

Cartridge

Laboratory instrument

Purifying target biomolecules, such as nucleic acids or proteins, from a biological source is a time intensive process and is typically performed by a skilled technician or scientist owing to the highly technical nature of the work. Systems, devices, and methods disclosed herein enable the automated bioprocessing and purification of target biomolecules from a biological source. For example, an instrument and disposable cartridge are provided for automatedly isolating and purifying nucleic acids (such as plasmid DNA from a bacterial culture) or for isolating protein from any biological sample. Such an exemplary instrument and cartridge can work in concert to timely release, mix, and move the target biomolecule and various reagents and buffers through a target biomolecule purification process, resulting in a purified target biomolecule with less manual oversight than traditional approaches.

Systems, methods, and devices for automated nucleic acid and protein isolation

The invention relates to methods of separating polynucleotides, such as DNA, RNA and PNA, from solutions containing polynucleotides by reversibly binding the polynucleotides to a solid surface, such as a magnetic microparticle.

Modified surfaces as solid supports for nucleic acid purification

The present invention relates to a gene that encodes a hyperactive reverse transcriptase having DNA polymerase activity and substantially reduced RNase H activity, vectors containing the gene and host cells transformed with the invention. The present invention also includes a method of producing the hyperactive reverse transcriptase, producing cDNA from mRNA using the reverse transcriptase of the invention, kits and assay templates made using the hyperactive reverse transcriptase.

Recombinant reverse transcriptases

The present invention provides novel algorithms for designing oligonucleotides that do not substantially hybridize to a small group of unwanted transcripts, while hybridizing to most other transcripts. Such oligonucleotides are particularly useful as primers for reverse transcription. The invention also provides compositions containing oligonucleotides that do not substantially hybridize to a small group of unwanted transcripts, while hybridizing to most other transcripts.

Reverse transcription primers and methods of design

This invention pertains to a method for generating a pool of nucleic acid fragments useful for in vitro recombination and the creation of novel DNA sequences that encode desirable proteins or enzymes. The invention provides a defined mixture of nucleic acids and methods for use in the synthesis, mutagenesis, and recombination of nucleic acids. Nucleic acids may be synthesized by creating a nucleic acid extension ladder, annealing the extension ladder to template nucleic acids, and further extending the ladder of nucleic acids. The invention also relates to methods for performing repeated cycles of synthesis for the purpose of mutagenesis or recombination, methods for producing mutant peptides and proteins from the mutagenized or recombined nucleic acids, and methods for selecting a peptide, polypeptide or protein having altered biological activities.

Methods for recombinatorial nucleic acid synthesis

Growing community of inventors

Austin, TX, United States of America

Robert Setterquist