Pacific Biosciences of California, Inc.

Robert Sebra

Stephen Wilson Turner

Jonas Korlach

Robin A Emig

Adrian Nielsen Fehr

Benjamin A Flusberg

Jeremy Gray

Stephen P Dudek

Keith P Bjornson

Mathieu Foquet

Fred C Christians

John S Eid

Jon M Sorenson

Lei Sun

Natasha Popovich

Ronald L Cicero

Hans Callebaut

Steven Lin

Bidhan P Chaudhuri

Gregory Kearns

Ravi Dalal

The invention relates to devices and methods for nanopore sequencing. The invention includes arrays of nanopores having incorporated electronic circuits, for example, in CMOS. In some cases, the arrays of nanopores comprise resistive openings for isolating the electronic signals for improved sequencing. Methods for controlling translocation of through the nanopore are disclosed.

Nanopore sequencing devices and methods

Methods for non-random loading of single analyte molecules into array structures are provided. The methods allow for distribution of a population of target molecules into a plurality of size confined regions such as wells. Sizing moieties are linked to individual target molecules. The sizing moieties are of sufficient size, relative to the size-confined reaction or observation regions, such that only a selected number of sizing moieties will fit into the size confined regions. The confined regions and the sizing moieties or target molecules comprise a selected charge that allow for controlling the loading of the sizing moities.

Single molecule loading methods and compositions

Methods, substrates, and devices related to arrays of optical confinements having surfaces with high levels of bias. Substrates having transparent or silica based portions and opaque or reflective portions are treated with 1) a selective passivating agent, that selectively coats the opaque or reflective regions, 2) a functionalizing agent such as a coupling agent, and 3) a selective removal agent, which selectively removes functionalizing agent from the passivated opaque or reflective surfaces.

Selectively functionalized arrays

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San Francisco, CA, United States of America

Robert Sebra