Merck Patent Gesellschaft MIT Beschrankter Haftung

Robert Schmucker

Wolfgang Hillen

Ulrike Guelland

A polypeptide having mutarotase activity is obtained from a host microorganism that has been transformed with a molecule having a recombinant DNA sequence. The molecule having a recombinant DNA sequence is prepared by removing the first 60 nucleotides of a DNA sequence originating from the genome of Acinetobacter calcoaceticus that codes for the polypeptide, modifying the following 21 nucleotides and fusing of the resultant structural gene with the start of the tetracycline-repressor gene and with an effective promotor sequence. The tetracycline-regressor gene and the promotor sequence preferably originate from the same microorganism such as E. coli in which expression of the polypeptide is carried out, and result in increased yield of the expressed polypeptide having mutarotase activity.

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Erlangen, Germany

Robert Schmucker