Biogen Ma Inc.

Ajinomoto Co., Ltd.

Samsung Bioepis Co., Ltd.

Robert S Gronke

Daisuke Takahashi

William F Kiesman

Xianglin Shi

Taisuke Ichimaru

Yannick Fillon

Héctor Zaanoni

Hien Nguyen

Xuan Zhou

Firoz Antia

Wuming Yan

Hong Jiang

Ratnesh S Joshi

Takuya Hamagaki

Orlando A Jaquez

Austen Ng

Songyoung Kim

Jeehoon Ghil

Hector Zaanoni

Orlando Jaquez

Yoonseok Lee

The present disclosure describes a convergent liquid phase process for manufacturing oligonucleotides by coupling two or more oligonucleotide fragments, each of which have two or more nucleotides. Also provided by the present disclosure are reaction steps involved the convergent liquid phase process.

Convergent liquid phase syntheses of oligonucleotides

The present invention relates to improved methods in the separation recombinant polypeptides with post-translational modifications from complex mixtures through the use of a cation exchange medium.

Use of cation-exchange chromatography in the flow-through mode to enrich post-translational modifications

The present invention relates to a method for PEGylating interferon beta.

Pegylation method

The invention herein describes a method to purify a target oligonucleotide using hydrophobic interaction chromatography (HIC). The method includes adding a salt to a mixture of the target oligonucleotide and product-related impurities, applying the diluted mixture, at a particular dynamic loading capacity, to the hydrophobic interaction chromatography resin (or hydrophobic adsorbent), washing the hydrophobic adsorbent with an aqueous salt solution, eluting the target oligonucleotide with a eluting solution, and collecting the eluent comprising the target oligonucleotide.

Hydrophobic interaction chromatography for purification of oligonucleotides

The present invention pertains to improved methods of using dye-ligand affinity chromatography for the isolation of antibodies or proteins comprising an antibody fragment (such as Fc fusion proteins) from a mixture of undesirable contaminants. In particular, the use of an organic polymer such as polyethylene glycol (PEG) in the elution phase of an antibody/dye-ligand chromatography isolation procedure results in improved separation of target antibodies from undesirable contaminants. The methods described herein are particularly useful in separating or removing antibody aggregates, misfolded antibodies, and virus contaminants from target antibodies.

Purification of immunoglobulins

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Boston, MA, United States of America

Robert S Gronke