Other

University of Cincinnati

Cryopharm

Robert S Franco

Murray Weiner

Raymond P Goodrich, Jr

Christine M Williams

Hans W Zimmer

Orlando J Martelo

Heterocyclic diones of the class containing 3-(heteroarylalkylene)- and 3(arylalkylene)-2, 4 (3H, 5H)-furandiones, and 3-(heteroarylalkylene)-pyrrolidene diones and -thiophene diones are disclosed as being effective cancer chemotherapy drugs, some of which are themselves novel compounds. Various members of this class were tested both in vitro and in vivo to demonstrate their effectiveness at reducing or inhibiting cancer tumor cells. The class of drugs was evaluated against a series of 10 human tumors in the in vitro Human Tumor Cell Assay procedure. The procedure indicates that this class of heterocyclic diones is effective against a broad variety of types of cancer and has little or no toxic effects based upon hematological and pathological evaluations.

3-(heteroarylalkyene)- and 3-(arylalkylene)-2,4(3H,5H)-heterocyclic

A method of introducing effectively non-anionic agents into mammalian red blood cells without unacceptable loss of cell contents, utilizing an osmotic pulse technique wherein an aqueous diluent medium is provided containing the desired effectively non-anionic agent(s) and an effective amount of water-soluble polyanion. The polyanion is non-disruptive to the lipid outer membranes of red blood cells and contains at least two anionic moieties per molecule. The method comprises suspending packed red blood cells in an aqueous solution containing a compound which readily diffuses into and out of the cells, rapidly creating a trans-membrane osmotic gradient by diluting the solution containing the cells in suspension with an essentially isotonic aqueous medium containing an effective amount of polyanion and one or more effectively non-anionic agents to be introduced, thereby causing diffusion of water into the cells with consequent swelling and increase in permeability of the outer membranes, and maintaining the increase in permeability for a period of time sufficient only to permit transport of the desired agent and polyanion into the cells and diffusion of the compound out of the cells. In another embodiment, for use where a polyanion in the aqueous diluent medium would be undesirable, the red blood cells are subjected to shear stress immediately after the osmotic pulse occurs, resulting in resealing of the outer membranes of the cells and regaining their original size and shape, with the desired agent(s) incorporated therein.

Method for introducing desired agents into red blood cells

A process and medium are disclosed for the lyophilization of red blood cells which comprises the use of solutions including monosaccharide hexoses and pentoses, biocompatible polymers and polyanions to permit the reconstitution of viable red blood cells.

Lyophilization of red blood cells

Method and apparatus for creating reversible intracellular hypertonicity in red blood cells of mammals in order to introduce desired materials into the cells or achieve therapeutically desirable changes in the characteristics of intracellular hemoglobin. A packed red blood cell fraction is incubated in a solution containing a compound which readily diffuses into and out of the cells; a trans-membrane osmotic gradient is rapidly created by contacting the cells with a near-isotonic aqueous medium with or without a desired agent to be introduced, causing diffusion of water into the cells with consequent swelling thereof and permitting diffusion of said compound out of the cells; and the cells are then separated and washed to obtain a sterile fraction of substantially intact cells.

Growing community of inventors

Cincinnati, OH, United States of America

Robert S Franco