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Biotechnica International, Inc.

Robert S Daves

Robert R Yocum

Michael C Chen

Scott Van Arsdell

Gregory T Maine

A DNA sequence having a yeast ENO2 promoter functionally fused to a heterologous gene.

Hybrid yeast promoter comprising an ENO2 UAS and TATA region and an

A vector having a gene for resistance to an antibiotic otherwise capable of killing a host yeast cell, the gene being transcribed from a yeast promoter sequence and the vector being capable of being integrated into a chromosome of the host yeast cell; and a diploid or greater ploidy yeast cell transformed by such a vector with heterologous DNA.

Industrial yeast comprising an integrated glucoamylase gene

A vector including a DNA sequence encoding a secretory signal sequence substantially identical to the secretory signal-encoding sequence of a glucoamylase gene from Saccharomyces diastaticus or S. cerevisiae; and upstream from the signal-encoding sequence, a DNA sequence capable of promoting transcription in yeast (e.g., a high yield promoter, such as the promoter of the triose phosphate isomerase gene), transcription of the signal-encoding sequence being under the control of the transcription-promoting sequence, a site for the insertion into the vector of a heterologous DNA sequence, in reading frame with the signal-encoding sequence. The vector is useful as an expression vector in yeast.

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Reading, MA, United States of America

Robert S Daves