Other

Abbott Laboratories Corporation

Biotechnica International, Inc.

Robert R Yocum

Robert S Daves

John J Carrino

Keith C Backman

Michael C Chen

George H Shimer, Jr

Gregory T Maine

Scott Van Arsdell

A vector having a gene for resistance to an antibiotic otherwise capable of killing a host yeast cell, the gene being transcribed from a yeast promoter sequence.

Yeast vectors conferring antibiotic resistance

A DNA sequence having a yeast ENO2 promoter functionally fused to a heterologous gene.

Hybrid yeast promoter comprising an ENO2 UAS and TATA region and an

The present invention involves methods of improving the Ligase Chain Reaction (LCR.TM.) amplification schemes by modifying at least one probe end so that the probability of the probe contributing to spurious ligation and signal development is greatly reduced. Only after specific hybridization of the modified probe with true target are the modified ends 'corrected' by endonuclease IV in a target dependent fashion to allow participation of the probe in the enzymatic ligation reaction. Specific modifications include 3' phosphate blocking groups and nucleic acid overhangs containing an abasic site at the point of ligation. Further embodiments include probes modified to contain ribonucleotide moieties which, after amplification, can be cleaved by RNase to destroy the amplification products and reduce the risk of contamination.

Ligase chain reaction with endonuclease IV correction and contamination

A vector having a gene for resistance to an antibiotic otherwise capable of killing a host yeast cell, the gene being transcribed from a yeast promoter sequence and the vector being capable of being integrated into a chromosome of the host yeast cell; and a diploid or greater ploidy yeast cell transformed by such a vector with heterologous DNA.

Industrial yeast comprising an integrated glucoamylase gene

A vector including a DNA sequence encoding a secretory signal sequence substantially identical to the secretory signal-encoding sequence of a glucoamylase gene from Saccharomyces diastaticus or S. cerevisiae; and upstream from the signal-encoding sequence, a DNA sequence capable of promoting transcription in yeast (e.g., a high yield promoter, such as the promoter of the triose phosphate isomerase gene), transcription of the signal-encoding sequence being under the control of the transcription-promoting sequence, a site for the insertion into the vector of a heterologous DNA sequence, in reading frame with the signal-encoding sequence. The vector is useful as an expression vector in yeast.

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Arlington, MA, United States of America

Robert R Yocum