Bloodcenter Research Foundation

The Medical College of Wisconsin, Inc.

The Blood Center of Southeastern Wisconsin

Versiti Blood Research Institute Foundation, Inc.

Robert R Montgomery

Thomas J Kunicki

Methods and kits for measuring levels of von Willebrand factor function in a sample without using a platelet aggregation agonist, such as ristocetin, comprising recombinant glycoprotein Ib&#x3b1; having a combination of G233V, D235Y and M239V mutations and an agent to detect a complex between the recombinant glycoprotein Ib&#x3b1; and von Willebrand factor.

Methods and kits for measuring von Willebrand factor

Methods and kits for measuring levels of von Willebrand factor function in a sample without using a platelet aggregation agonist, such as ristocetin, comprising recombinant glycoprotein Ib&#x3b1; having at least two of a G233V, D235Y and M239V mutations and an agent to detect a complex between the recombinant glycoprotein Ib&#x3b1; and von Willebrand factor.

Methods and kits for measuring von willebrand factor

A method for maintaining intact, non-degraded von Willebrand factor by preventing the action of calcium activated protease(s) responsible for degradation of the factor. The action of the calcium activated protease(s) may be avoided by removing the blood platelet source of the protease(s), by filtering or centrifugal separation, or by inactivating the protease with a chelating agent removing the calcium, by a protease inhibitor, or an alkylating agent.

Growing community of inventors

Mequon, WI, United States of America

Robert R Montgomery