Chiron Corporation

Other

Novartis Vaccines & Diagnostics, Gmbh

Robert O Ralston

Michael Houghton

George Kuo

Kent B Thudium

John Andrew Hall

Frank I Marcus

Barbara A Gervase

Kim M Berger

Qui-Lim Choo

Mark A Goldsmith

John E Murphy

Oui-Lim Choo

Mohammad Ayub Khan

Two Hepatitis C Virus envelope proteins (E1 and E2) are expressed without sialylation. Recombinant expression of these proteins in lower eukaryotes, or in mammalian cells in which terminal glycosylation is blocked, results in recombinant proteins which are more similar to native HCV glycoproteins. When isolated by GNA lectin affinity, the E1 and E2 proteins aggregate into virus-like particles.

Antibodies to hepatitis C virus asialoglycoproteins

Hepatitis C virus asialoglycoproteins

Cells producing recombinant retroviral particles are provided. The cells contain a first vector having a coding region encoding retroviral LTRs and a packaging signal under the control of an expression control system, a tRNA binding site located upstream from the packaging signal and origin of second strand DNA synthesis located downstream from the packaging signal. The cells also contain a second vector having a coding region encoding retroviral capsid proteins gag and pol under the control of an expression control system and a third vector having a coding region encoding a simian type D retrovirus envelope glycoprotein under the control of an expression control system.

Retroviral vectors pseudotyped with SRV-3 envelope glycoprotein sequences

Host cells may be treated for an infection or a hyperproliferative disorder which is characterized by the presence, in the affected cells, of a trans-acting factor capable of regulating gene expression by inserting into the cells a polynucleotide construct having a cis-acting regulatory sequence which is regulated by the trans-acting factor and an effector gene which renders said cell susceptible to protection or destruction. For example, the cis-acting region may be homologous to the HIV tar region, and the effector gene may encode ricin A or HSV-1 thymidine kinase. Upon infection with HIV, the HIV tat protein activates the tar region, and induces transcription and expression of ricin A, resulting in cell death, or of HSV-1 tk, resulting in cell death upon treatment with dideoxynucleoside agents such as acyclovir and gancyclovir.

Methods and polynucleotide constructs for treating host cells for

Growing community of inventors

Danville, CA, United States of America

Robert O Ralston