Epoch Biosciences, Inc.

Elitech Holding B.v.

Epoch Pharmaceuticals, Inc.

Blood Cell Storage, Inc.

Elitech Holdings B.v.

Robert O Dempcy

Nicolaas M J Vermeulen

Alexander A Gall

Irina Aleksandrovna Afonina

Igor V Kutyavin

Eugeny Alexander Lukhtanov

Michael W Reed

Yevgeniy S Belousov

Michael J Singer

Alexei V Vorobiev

Steven J Geelhood

A David Adams

Karen M Singer, Legal Representative

Nicolaas MJ Vermeulen

Alkyl amine dyes, oligonucleotide probes prepared from the alkyl amine dyes, phosphoramidites and solid supports prepared from the alkyl amine dyes, and methods of labeling biological agents using the alkyl amine dyes.

Alkyl amine compounds for fluorescent labeling

Fluorescent pH detector and methods for measuring pH using the fluorescent pH detector.

Fluorescent pH detector system and related methods

Oligonucleotide probes containing two labels are provided and are useful in hybridization assays. The probes can also contain a minor groove binding group.

Fluorescent quenching detecting reagents and methods

Methods for designing an oligonucleotide sequence having a selected duplex stability are provided wherein the oligonucleotide portion comprises at least one modified base selected from universal bases, unsubstituted and 3-substituted pyrazolo[3,4-d]pyrimidines and 5-substituted pyrimidines, and optionally having attached minor groove binders and reporter groups.

TM leveling methods

Methods and probes are provided for the analysis of target sequences having two or more polymorphisms wherein one of the polymorphisms is to be distinguished and another polymorphism is to be masked.

Single nucleotide polymorphism analysis of highly polymorphic target sequences

Software systems and methods for predicting the melting temperature (Tm) and other characteristics of oligonucleotides, including modified oligonucleotides. Modified oligonucleotides are provided containing bases selected from unsubstituted and 3-substituted pyrazolo[3,4-d]pyrimidines and 5-substituted pyrimidines, and optionally have attached minor groove binders and reporter groups.

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Fluorescent quenching detection reagents and methods

Oligonucleotide probes/conjugates are provided along with method for their use in assays to monitor amplification wherein the signal produced does not rely on 5' nuclease digestion.

Real-time linear detection probes: sensitive 5'-minor groove binder-containing probes for PCR analysis

Modified oligonucleotides are provided containing bases selected from unsubstituted and 3-substituted pyrazolo[3,4-d]pyrimidines and 5-substituted pyrimidines, and optionally have attached minor groove binders and reporter groups.

Compositions and methods for fluorescent detection of nucleic acids are provided. The compositions can be detected by fluorescence when hybridized to a nucleic acid containing a target sequence, but are non-fluorescent in the non-hybridized state. Alternatively, the fluorescence properties of the compositions change in a detectable manner upon hybridization to a nucleic acid containing a target sequence. Methods for synthesis and methods of use of the compositions are also provided.

Hybridization-triggered fluorescent detection of nucleic acids

Modified oligonucleotides for mismatch discrimination

The invention provides compositions and methods for improved hybridization analysis utilizing DNA, RNA, PNA and chimeric oligomers in which one or more purine bases are substituted by a pyrazolo[5,4-d]pyrimidine or by a 7-deazapurine purine analogue. Reduced self-aggregation and reduced fluorescence quenching are obtained when the oligomers are used in various methods involving hybridization. Methods of synthesis, as well as novel synthetic precursors, are also provided.

Non-aggregating non-quenching oligomer comprising nucelotide analogs, method of synthesis and use thereof

The present invention provides a nucleoside comprising a pyrazolopyrimidine base and a process for producing the same. In particular, the processes of the present invention comprises using a halogenated pyrazolopyrimidine base and removing the halogen after the base is coupled to a sugar moiety. The presence of the halogen on the nucleoside base allows facile and economical production of a large quantity of nucleosides.

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Kirkland, WA, United States of America

Robert O Dempcy