Portland State University

Louisiana State University and Agricultural & Mechanical College

Women & Infants' Hospital

Robert Michael Strongin

Martha Sibrian-Vazquez

Jorge Omar Escobedo-Cordova

Jorge O Escobedo Cordova

Mark Allen Lowry

Jialu Wang

Lei Wang

Grover L Waldrop

David R Amspacher

Marcelo C Saraiva

Xiaofeng Yang

Nadia N St Luce

Rakesh Kumar Singh

Isiah Manuel Warner

Satyan Kalkunte

Laurent Brard

Jonathan J Abramson

Youjun Yang

Mark Lowry

Sayo O Fakayode

Lovemore Hakuna

Xiangyang Xu

Jorge Omar Escobedo Córdova

Onur Alpturk

Claude Joseph Davis

Weihua Wang

Larry Allen Cabell

Yixing Guo

Ming He

Oleksandr Rusin

Patrick T Lewis

Tendai Mafireyi

Jorge O Escobedo-CÓRDOVA

Seminaphthofluorophore-selenium probes are disclosed. The probes include a seminaphthofluorophore scaffold substituted with at least one diselenide moiety having a formula —Y—C(Q)O—(CH)—Se—Se—(CH)—OR, where Ris hydrogen or C-Calkyl, Q is O or S, Y is O or N(R) where Ris H or alkyl, and m and n independently are integers. The probes may be used to detect presence of a thioredoxin reductase.

Seminaphthofluorophore-selenium probes for thioredoxin reductase

Embodiments of methods and compounds for isolating and detecting lysophosphatidic acids (LPAs) are disclosed. Kits for performing the methods also are disclosed. LPAs are isolated from biological samples by liquid-liquid extraction followed by solid phase extraction. LPA species may be separated by HPLC, and the separated species may be identified and quantified. Also disclosed are embodiments of compounds capable of universally detecting a plurality of LPA species with substantially equivalent sensitivity. Embodiments of the disclosed compounds are useful for determination of total LPA concentration in a sample comprising a plurality of LPA species without separation of individual LPA species.

Lysophosphatidic acid detection

Molecular probes for detecting and imaging pancreatic cancer are disclosed. The probes are modified benzoxanthene fluorophores, which are selectively taken up by pancreatic cancer cells, such as pancreatic ductal adenocarcinoma cells. Embodiments of the disclosed probes are useful for pancreatic cancer detection, therapeutic monitoring, and/or image-guided surgery.

Molecular probes for detection and imaging of pancreatic cancer

Embodiments of templated polymeric materials capable of binding lysophosphatidic acids (LPAs) are disclosed. Methods of making and using the templated polymeric materials also are disclosed. The disclosed templated polymeric materials are molecularly imprinted polymers that bind LPAs and facilitate the production of lysophosphatidic acid-enriched samples, for instance through extraction of lysophosphatidic acids from biological samples, such as plasma or serum samples.

Enrichment of lysophosphatidic acids with templated polymeric materials

Embodiments of compounds for selectively detecting an analyte are disclosed, along with methods and kits for detecting analytes with the compounds. The compounds are bridged viologen conjugates including at least one fluorophore according to the general structure  At least one of R/R, R/R, R/R, R/R, R/R, and/or R/Rtogether form a substituted or unsubstituted cycloalkyl or aryl.

Analyte detection using near-infrared fluorophores

Embodiments of near-infrared (NIR) dyes are disclosed, along with methods and kits for detecting analytes with the NIR dyes. The NIR dyes have a structure according to the general structure At least one of R/R, R/R, R/R, R/R, R/R, and/or R/Rtogether forms a substituted or unsubstituted cycloalkyl or aryl.

Near-infrared fluorescent dyes with large stokes shifts

Embodiments of probes for selectively detecting compounds having a thiol group and an amino group, e.g., cysteine and/or homocysteine, are disclosed, along with methods and kits for detecting the compounds in neutral media with the probes. The probes have a structure according to the general formula where R-Rindependently are hydrogen hydroxyl, halogen, thiol, thioether, lower aliphatic, or lower alkoxy, x is an integer from 0 to 4, and each Rindependently is halogen, hydroxyl, thiol, thioether, lower aliphatic, or lower alkoxy. Embodiments of the disclosed probes are capable of undergoing condensation/cyclization reactions with cysteine and/or homocysteine. Cysteine and/or homocysteine can be selectively detected and identified by determining fluorescence emission of the probes at characteristic wavelengths.

Fluorescence detection of cysteine and homocysteine

Probes for selectively detecting compounds comprising a thiol group and an amino group ('thiols') are disclosed. Exemplary thiols include cysteine, homocysteine, and glutathione. Embodiments of the disclosed probes produce a detectable change in absorbance spectrum and/or emission spectrum when reacted with one or more thiols in solution. Methods and kits for performing the detection also are disclosed. The probes have a general formula where each bond depicted as '' is a single or double bond; R, R-Rand Rindependently are hydrogen, hydroxyl, thiol, lower alkyl, carboxyalkyl, amino, alkoxy, or halogen; Ris an &#x3b1;,&#x3b2;-unsaturated aliphatic ester; Ris oxygen, sulfur, hydrogen, hydroxyl, thiol, lower alkyl, carboxyalkyl, amino, alkoxy, or halogen, or Rand Rtogether form a cycloalkyl or aryl ring; Xis CH, S, NH, O, Se, Si(CH), Ge(CH), Sn(CH), or C(CH); and Xis CH, CH, N, NH, or CRwhere Ris aryl.

Selective detection of amino group containing thiols

Colorimetric and fluorometric methods are disclosed for the rapid, accurate, selective, and inexpensive detection of homocysteine, or of homocysteine and cysteine, or of cysteine. The methods may be employed with materials that are readily available commercially. The novel methods are selective for homocysteine, for cysteine, or for total homocysteine and cysteine, and do not cross-react substantially with chemically-related species such as glutathione. The homocysteine-selective method does not have substantial cross-reactivity to the very closely related species cysteine. The cysteine-selective method does not have substantial cross-reactivity to the very closely related species homocysteine. The methods may be used, for example, in a direct assay of human blood plasma for homocysteine levels.

Colorimetric and fluorometric determination of homocysteine and cysteine

The present teachings provide compounds of Formulae I and II: and pharmaceutically acceptable salts, hydrates, complexes, esters, and prodrugs thereof, wherein R, R, R, R, R, R, and X are as defined herein. The present teachings also provide methods of making the compounds of formulae I and II, and methods of treating RyR-associated conditions, disorders, and diseases that include administering a therapeutically effective amount of a compound of formula I or II to a subject in need thereof. In addition, the present teachings relate to methods of reducing the open probability of a ryanodine receptor, and methods of reducing Carelease across a ryanodine receptor (e.g., into the cytoplasm of a cell), by contacting a compound of formula I or II with a ryanodine receptor. 

Compounds and methods for modulating activity of calcium release channels

Embodiments of compounds for selectively detecting a thiol are disclosed. In some embodiments, the compounds are bridged viologens, and the compounds are capable of reacting with homocysteine and/or glutathione in a buffered solution to produce a change in the solution's absorbance spectrum and/or emission spectrum. Also disclosed are embodiments of methods and kits for detecting homocysteine and/or glutathione with the disclosed bridged viologens.

Thiol detection

Xanthene compounds are disclosed having fluorescence at multiple wavelengths. Also disclosed are methods for their synthesis and use. Some of the compounds fluoresce at three wavelengths, emitting white light. Uses include the imaging of biological tissues, illumination, and display technologies. Many of the compounds have large Stokes shifts, and are resistant to photobleaching. The fluorescence may be readily distinguished from that of endogenous fluorophores, and from that of most existing, commercially-available fluorescent probes. The compounds are well suited for use in 'multiplexing' techniques. They exhibit clear isosbestic and isoemissive points, and have broad absorption and emission ranges. 

Fluorescent xanthenes and white light fluorophores

Growing community of inventors

Portland, OR, United States of America

Robert Michael Strongin