Baylor Research Institute

Other

Baylor Research Foundation

Diagnostic Reagents, Inc.

Robert M Dowben

J Lester Matthews

Edward Forest

Kirpal S Gulliya

Shazib Pervaiz

Joseph T Newman

James R Bunting

Jospeh T Newman

A therapeutic composition derived from a pre-activated photoactive compound and a conveyor for destroying tumor or other pathogenic biological contaminants infecting animal body tissues. The conveyor can either be a matrix support or an antibody. The activation of the photoactive compound to produce the pre-activated photoactive compound is carried out by the use of an activating agent. The pre-activated photoactive compound retains its therapeutic activity subsequent to activation. A method for preparing such a therapeutic composition is disclosed. Methods for treating animal body tissue, either outside the body or inside the body, infected with tumors or other pathogenic biological contaminants are disclosed.

Method of treating with therapeutic composition comprising photoactive

Therapeutic compositions derived from photoactive compounds

A pre-activated therapeutic agent derived from a photoactive compound for destroying tumor or other pathogenic biological contaminants infecting animal body tissues. The activation of the photoactive compound is carried out prior to the photoactive compound is being brought into contact with the tissue to be treated. The resultant pre-activated therapeutic agent, or mixture, retains its therapeutic activity subsequent to activation. A method for preparing such a pre-activated therapeutic agent is disclosed. Methods for treating animal body tissue, either outside the body or inside the body, infected with tumors or other pathogenic biological contaminants are disclosed.

Pre-activated therapeutic agents derived from photoactive compounds

An ultraviolet light beam is split into two beams which are directed toward a reference cell and a sample cell, respectively. A mechanical rotating light chopper permits the reference and sample cells to be alternately illuminated. Radiation from the reference and sample cells is directed toward and sensed by a single photomultiplier tube. An up/down counter counts the number of photons impinging on the photomultiplier tube from the reference and sample cells. Photodiodes in conjunction with the mechanical light chopper cause the up/down counter to count up when photons are arriving from the sample cell and cause the up/down counter to count down when photons are arriving from the reference cell. A source intensity monitor prohibits further measurements after a preselected amount of incident flux has been sensed. The intensity monitor includes a photodetector circuit which senses a portion of the incident light. Pulses from the detector are fed to a counter and the number in the counter is compared to a preselected number which is set on a thumbwheel switch. When the comparison condition is met, the up/down counter of the fluorimeter is stopped.

Photon counting fluorimeter

Improved methods for determining very low concentrations of substances present in fluid samples are provided by employing light emitting tracer compounds and (1) counting the photons emitted therefrom while discriminating against noise, nonspecific light, and quenching effects of the sample, or (2) counting the photons emitted therefrom over a predetermined integrated light flux, or a combination of (1) and (2). Further, novel fluorescently labeled low molecular weight antigens are provided which can be employed in competitive binding techniques in which the above described photon counting methods are useful. A homogeneous competitive binding assay, employing photon emitting tracer materials, which eliminates the need for separating bound from unbound materials is also provided. Finally, a modified enzyme amplification technique is set forth employing enzymes active in the bound phase to provide assay techniques useful for extremely low concentration assays.

Methods for performing chemical assays using fluorescence and photon

Growing community of inventors

Dallas, TX, United States of America

Robert M Dowben