Hoffmann-la Roche Inc.

The United States of America As Represented by the Department of Health

Other

Robert M Crowl

Robert C Gallo

Flossie Wong-Staal

George M Shaw

E Prem Reddy

Ram S Bhatt

Daru Young

Mohindar S Poonian

Kenneth J Collier

An envelope protein of the etiologic agent of acquired immune deficiency syndrome (AIDS) and a method for its preparation are disclosed. Proviral DNA is transferred into a host cell after engineering into an expression vector which produces the envelope protein. A method of testing human blood for the presence of antibodies to the AIDS virus using the AIDS envelope protein is disclosed.

Acquired immune deficiency syndrome (AIDS) viral envelope protein and

Methods and compositions are provided for the cloning and expression of plasmids bearing genes encoding a novel protein derived from HTLV-III. This protein, which is called the gag/env protein and which contains antigenic determinants from both the core and envelope proteins of HTLV-III, can be purified to homogeneity and used as the basis for diagnostic tests to detect the presence of antibodies against viruses associated with AIDS or the viruses themselves in human sera and other biological fluids. The gag/env protein may also be formulated for use as a vaccine for protection against AIDS through prophylactic immunization.

Expression and purification of an HTLV-III gag/env gene protein

Th preparation of a double-stranded polydeoxy ribonucleotide having cohesive termini each comprising one strand of a double-strand restriction endonuclease recognition site and, between the termini, a structural gene coating for the expression of recombinant growth hormone releasing factor, at least a majority of the codons and the coding strand of said gene being codons preferred for the expression of microbial genomes; a method of producing such DNA a recombinant microbial cloning vehicle comprising such DNA and the novel methods of producing said peptide are described.

Recombinant growth hormone releasing factor

Improved vectors and methods for regulating the expression in bacteria of a eucaryotic anti-viral protein, such as mature human immune interferon, the gene for which has been cloned onto a bacterial plasmid, is disclosed. The improved vectors incorporate and method utilizes transcriptional regulatory elements derived from bacteriophage lambda and ribosome binding sites either derived from bacteriophage lambda and/or synthesized chemically.

Novel vectors and method for controlling interferon expression

Growing community of inventors

Cedar Grove, NJ, United States of America

Robert M Crowl