Wisconsin Alumni Research Foundation

University of California

University of Kentucky Research Foundation

Other

Robert M Corn

Hye Jin Lee

Kenneth F Haynes

Anthony G Frutos

Michael F Potter

Catherine Loudon

Jennifer M Brockman

Megan Szyndler

Stephen C Weibel

Eric P Codner

Voula Kodoyianni

Alastair William Wark

Lloyd M Smith

Robert John Hamers

Bernard Weisblum

Robert M Goodman

Brian L Frey

Michael Robert Shortreed

Andrew T Ulijasz

Matthew G Erickson

Bryce P Nelson

Mark R Liles

Claire E Jordan

Steven E Kornguth

Gabriel Loget

Terry T Goodrich

Matthew R Lockett

Emily A Smith

Han Wai M Fung

Bin Sun

Matthew J Wanat

Kendra Frederick

Mana Toma

Michael J Green

Tony G Frutos

Novel devices and methods of capturing, controlling and preventing infestation of insects using microfabricated surfaces are provided. In particular, a mechanism of insect capture inspired by the microstructures of the leaf surfaces of plants and the key features of those surfaces with respect to the capture and control of pests have been determined and engineered into a variety of microfabricated surfaces capable of reproducing the effectiveness of these physical capture methods.

Microfabricated surfaces for the physical capture of insects

The present invention relates to nanocones and nanomaterials. In one embodiment, the present invention provides a method of fabricating an array of nanostructures on a flexible film, comprising self-assembling a layer of particles on a film, and fabricating an array of nanostructures by etching and/or modifying the film. In another embodiment, the present invention provides a microarray comprising a nanomaterial comprising a film configured for an array of one or more nanocones.

Nanostructured arrays on flexible polymer films

SPR-compatible substrates for high density microarray fabrication and analyses are provided. Novel carbon-on-metal thin film substrate architecture permits the integration of surface plasmon resonance detection with photolithographically fabricated biomolecule arrays for the analysis of biomolecular interactions. The utility of the technology is shown in the analysis of specific DNA-DNA, DNA-RNA and DNA-protein binding interactions. These new substrates may be used to determine the secondary structure of RNA molecules, to probe the sequence-specific binding kinetics and affinity of proteins and small molecules, and as substrates for small-molecule combinatorial chemistry platforms for drug discovery applications.

Surface plasmon resonance compatible carbon thin films

A substrate for testing a carrier liquid for biomolecules using SPR or other techniques provides a metallic island surrounded by a hydrophobic layer, the islands being a location for the attachment of probe molecules. The hydrophobic layer may also be a dielectric material providing improved sensitivity in SPR imaging.

Versatile substrate for SPR detection

Disclosed is a method for fabricating 1-dimensional micro-arrays using parallel micro-fluidic channels on chemically-modified metal, carbon, silicon, and/or germanium surfaces; a &#x3bc;L detection volume method that uses 2-dimensional nucleic acid micro-arrays formed by employing the 1-dimensional DNA micro-arrays in conjunction with a second set of parallel micro-fluidic channels for solution delivery, and the 1-dimensional and 2-dimensional arrays used in the methods. The methodology allows the rapid creation of 1- and 2-dimensional arrays for SPR imaging and fluorescence imaging of DNA-DNA, DNA-RNA, DNA-protein, and protein-protein binding events. The invention enables very small volumes necessary for a variety of bioassay applications to be analyzed by SPR. Target solution volumes as small as 200 pL can be analyzed.

Surface plasmon resonance imaging of micro-arrays

A surface plasmon resonance imaging apparatus provides an improved optical assembly allowing fixed source and detector operating with a horizontal test surface for a more compact design. In a preferred embodiment, a mechanical linkage of planar mirrors provides a single point adjustment of angle of incidence and angle of refraction while maintaining a constant optical axis of the source and detector.

Horizontal surface plasmon resonance instrument with improved light path

A surface plasmon resonant device provides practical portable operation through the use of a low power high efficiency LED source and a high-efficiency prism sample cell pre-loaded with probe molecules and sealed for field use. A simple mechanical control allows adjustment of angulation of the light and camera for accurate response outside of the laboratory.

Portable surface plasmon resonance imaging instrument

Disclosed are methods for making surface plasmon resonance-capable arrays wherein molecules, such as proteins or nucleic acids, or cells, are adhered to a metal substrate. The metal substrates are modified by depositing an &#x3c9;-modified alkanethiol monolayer to the substrate and then contacting the &#x3c9;-modified monolayer with a heterobifunctional linking compound. Biomolecules or cells can then be attached to the heterobifunctional linking compound. Also disclosed are arrays wherein glutathione-containing molecules are immobilized on the substrate and GST-containing molecules are then specifically immobilized onto the substrate, taking advantage of the affinity between glutathione and GST.

Fusion protein arrays on metal substrates for surface plasmon resonance imaging

Disclosed is a process to construct multi-component biomolecule or cellular arrays suitable for use in SPR imaging studies of large molecule, cellular/molecular, and cell/cell interactions. Also disclosed are the resulting arrays. The success of the procedure hinges on the use of a reversible protecting group to modify reversibly &#x3c9;-functionalized alkanethiols self-assembled on metal substrates. The arrays themselves include a metal substrate, a continuous layer of an identical &#x3c9;-modified alkanthiol adhered to the metal substrate, and one or more discrete spots of biomolecules or cells directly bonded to the continuous layer of &#x3c9;-modified alkenthiol. The areas of the continuous layer of &#x3c9;-modified alkenthiol not covered by one of the discrete spots are covered by a background material resistant to non-specific protein binding.

Biomolecule and/or cellular arrays on metal surfaces

Disclosed is a method to detect unlabeled nucleic acids (DNA and/or RNA) in a taxa, species, and organelle-specific fashion using surface plasmon resonance (SPR) imaging. Taxa-specific, species-specific, or organelle-specific nucleic acids are affixed to an SPR-suitable substrate. A nucleic acid sample to be analyzed is then contacted with the SPR-substrate and the substrate analyzed to determine the presence or absence of specific hybridization between the nucleic acids bound to the substrate and the nucleic acids contained in the sample. The method does not require that either the bound nucleic acids nor the sample nucleic acids be labeled. The method can be used to identify the source of nucleic acids, their sequence, as well as to identify organisms and place them within a given taxonomic hierarchy.

Label-free detection of nucleic acids via surface plasmon resonance

Disclosed is a process to construct multi-component biomolecule or cellular arrays suitable for use in SPR imaging studies of large molecule, cellular/molecular, and cell/cell interactions. The success of the procedure hinges on the use of a reversible protecting group to modify reversibly &ohgr;-functionalized alkanethiols self-assembled on metal substrates.

Biomolecule and/or cellular arrays on metal surfaces and product produced thereby

Adsorption of molecules onto a thin metallic surface such as of gold, silver or copper is measured by surface plasmon resonance (SPR) using Fourier Transform (FT) spectroscopy. Reflectance spectra from a prism/metallic film sample surface at a fixed angle of incidence is measured with FT spectroscopy. The reflectance spectrum exhibits a pronounced minimum due to the SPR effect, which can be shifted by changing the angle of incidence or metallic film thickness. The position of the reflectance minimum shifts in wavelength with the adsorption of molecules onto the gold surface due to a change in the index of refraction at the interface. The FT-SPR sensor instrument provides wavelength stability and reproducibility of the resonance wavelength to permit detection of small wavelength shifts, and also substantially increases the spectral range over which the SPR measurements can be made. A beam of broadband radiation from a Michelson interferometer is directed through an SPR sample cell and onto a detector where the output signal is processed using standard FT techniques.

Fourier transform surface plasmon resonance adsorption sensor instrument

Growing community of inventors

Madison, WI, United States of America

Robert M Corn