American Monitor Corporation

Robert L Long

Jerry W Denney

A colorimetric assay method and stabilized working reagents for the determination of magnesium in which an alkaline reagent containing a dye to complex magnesium reacts directly with the sample, requiring no protein-removal steps and no addition of substances to artificially correct for absorption differences of the metallized and free dye due to the presence of protein. The dye is either Calmagite or Eriochrome Black T. The reagent is buffered so that the pH is greater than about 9.0; the relative Van Slyke buffer value .beta.' of the reagent is greater than 1.

Magnesium assay with calmagite or eriochrome black T reagents

A diagnostic assay and reagent system for the determination of uric acid directly on a non-deproteinized sample of a biological fluid using an alkaline phosphotungstate reduction reaction in the presence of at least one organic sulfhydryl-containing compound.

Uric acid assay and reagent system therefor

An alkaline picrate reagent system useful for the quantitation of creatinine in biological fluids. Interference from constituents normally found in serum or urine is eliminated by the use of dimethylsulfoxide with preferably an aralkane detergent in combination with a kinetic or reaction rate analysis mode. It obviates the need for any pretreatment of the sample, and avoids the need of other chemicals in the system which if allowed to contaminate glassware would have a deleterious effect on other analyses performed in the laboratory.

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Indianapolis, IN, United States of America

Robert L Long