Waters Investments Limited

Robert Karol

Michael J Leveille

Jeffrey W Finch

Joseph M DeLuca

Daniel Wall

A method and apparatus for the continuous deposition of RP HPLC eluent, from isocratic or gradient elutions, on to a MALDI target using a parallel sample desalting method, allows increased sensitivity of the MALDI sample by pre-concentration of the analyte, separation of interfering contaminants from the analyte and increased dynamic range of peptide abundance that can be analyzed. The parallel processing of sample material allows greater throughput than that of manual methods.

Parallel concentration, desalting and deposition onto MALDI targets

A chromatographic flow cell having the capacity to handle fluid flow from one or more chromatographic systems, at widely varying flow rates without the need to change flow cells or detectors. The flow cell itself acts as a tee where two or more fluidic inlet lines intersect with a common inlet channel. The common inlet channel is configured so that the diameter and length minimizes the total volume, unswept volume, bandspreading, and backpressure. The common outlet channel, inlet lines and the outlet line are sized to provide for adequate fluidic handling at the varied flow rates.

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Marlboro, MA, United States of America

Robert Karol