Envirogen, Inc.

Shaw Intellectual Properties Holding, Inc.

Shaw Environmental and Infrastructure, Inc.

Robert Jon Steffan

Kevin Rock McClay

Charles Whitman Condee

Mary F DeFlaun

Jennifer Diane Michelson

Kevin R McClay

Simon Vainberg

The compounds disclosed herein are isoxazole derivatives that are useful as antimicrobial compounds, particularly as anti-bacterial compounds. The disclosed methods comprise incubating at least two different substrates in the presence of at least one oxygenase to provide the disclosed compounds, or to prepare and identify compounds that have antimicrobial activity.

Antimicrobial compounds

A method for converting alkenes into epoxides and, particularly, to convert alkenes into enantio-specific epoxides by the use of enzymes which may be in their naturally-occuring (native) form or in mutated form, such as a native or mutated non-haem diiron-containing monooxygenase, and novel compounds produced thereby.

Preparation of enantio-specific epoxides

A method for degrading an undesirable ether-based environmental contaminant by contacting the ether with a hydrogen-oxidizing microorganism to convert the ether to innocuous compounds which are environmentally acceptable, including treating the ether-based contaminants in situ or removing them from the contaminated site for treatment in a bioreactor, examples of the ether-based contaminants being tertiary butyl ethers of the type utilized as gasoline oxygenates, for example, methyl tert-butyl ether, ethyl tert-butyl ether, and methyl tert-amyl ether, and also ether solvents, for example, tetrahydrofuran.

Biodegradation of gasoline oxygenates

Disclosed is a method for monitoring the degradation of methyl tert-butyl ether in a contaminated media which contains methyl tert-butyl ether and/or which is treated under conditions which cause the degradation of methyl tert-butyl ether into 2-hydroxy isobutyric acid comprising: (A) evaluating the treated contaminated media to determine the absence or presence therein of 2-hydroxy isobutyric acid; (B) based on such evaluation, determining whether the conditions should be modified; and (C) modifying such conditions as may be necessary. Disclosed also is a method for degrading an ether comprising contacting the ether with a microorganism that is effective in oxidizing propane or isopropanol and 2-hydroxy-isobutyric acid.

Biodegradation of the gasoline oxygenates

Saturated aliphatic halocarbons, including environmental contaminants, are degraded to innocuous, environmentally acceptable compounds by contact, either in situ or in a bioreactor, with microorganisms that produce aromatic oxygenases, preferably with use of a co-substrate, for example, phenol, toluene, benzene, ethylbenzene and xylene, including the provision of novel bacteria that produce aromatic oxygenases, and new recombinant microorganisms that contain cloned aromatic oxygenase genes, examples of saturated aliphatic halocarbon that may be degraded to innocuous compounds being chloroform; bromoform; 1,2-dichloroethane; 1,2-dibromoethane; monochloroethane and monobromoethane.

Biological conversion of organic compounds

The present invention is directed to a method for degrading an undesirable ether-based environmental contaminant by contacting the ether with a propane-oxidizing microorganism or with an isopropanol-oxidizing microorganism to convert the ether to innocuous compounds which are environmentally acceptable, including treating the ether-based contaminants in situ or removing them from the contaminated site for treatment in a bioreactor. Examples of ether-based compounds which can be degraded are tertiary butyl ethers of the type utilized as gasoline oxygenates, for example, methyl tert-butyl ether, ethyl tert-butyl ether, and methyl tert-amyl ether and also ether solvents, for example, tetrahydrofuran.

A process for degrading volatile organic contaminants such as trichloroethylene. and remediating volatile organic contaminant contaminated media using trichloroethylene induced toluene monooxygenase activity in Pseudomonas mendocina KR-1 is described.

Method of degrading volatile organochlorides and remediation thereof

The present invention features methods and apparatus for processing waste material held in an aqueous medium. A sorbent is added to the aqueous medium. The sorbent reduces the effective concentration of waste material allowing biodegration of the waste material in solution. A preferred sorbent is rubber derived from ground or shredded tires.

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Wrightstown, PA, United States of America

Robert Jon Steffan