General Electric Company

Lockheed Martin Corporation

Ge Healthcare Bio-sciences Corp.

Other

Molecular Optoelectronics Corporation

Digital Pathco, LLC

Leica Microsystems Cms Gmbh

Global Life Sciences Solutions USA LLC

Robert John Filkins

Peter William Lorraine

Marc Dubois

Thomas E Drake

Matthew Christian Nielsen

Anup Sood

John Broddus Deaton, Jr

Samhita Dasgupta

Kashan Ali Shaikh

Brian Lee Lawrence

Alex David Corwin

Jun Xie

Glenn Scott Claydon

Ali Can

Kristina H Hedengren

Glenn A Forman

William P Kornrumpf

Michael Evans Graham

Siavash Yazdanfar

Harvey E Cline

Alok Mani Srivastava

Holly Ann Comanzo

Bin Wei

Joseph Alfred Iannotti

Bruno Hans Haider

Ge Wang

Kevin George Harding

Eugene Pauling Boden

Prabhjot Singh

Victor Petrovich Ostroverkhov

Kwok Pong Chan

Thomas James Batzinger

Subhrajit Roychowdhury

Michael John Gerdes

Matthias Hoebel

Xiaolei Shi

Arunkumar Natarajan

Glen Peter Koste

John Scott Price

Wenxiang Cong

Vasile Bogdan Neculaes

Dashan Gao

Jens Rittscher

Anthony Sebastian Bauco

David Gilles Gascoyne

Richard S Zhang

Rui Chen

Joseph Manak

Bruce Matthew Dunham

Donnell Eugene Crear

Kathryn Lynn Longley

Michael Christopher Montalto

Maximilian Lewis Seel

Kevin Bernard Kenny

James Paul Fulton

Zhengyu Pang

Thadd Clark Patton

Barbara Venchiarutti

Thomas Charles Adcock

Min-Yi Shih

Felix Martin Gerhard Roerig

Juan Borja

Edward James Nieters

Harry Israel Ringermacher

Todd Ryan Tolliver

John D Young

Fiona Ginty

Nannan Chen

Carl Stephen Lester

Krenar Tasimi

Robert William Tait

Juntao Wu

Gil Abramovich

Dirk Ryan Padfield

Michael Scott Lamphere

Evgenia Mikhailovna Kim

Thomas Etter

Ahmad Nadeem Ishaque

Lakshmi Sireesha Kaanumalle

Colin Craig McCulloch

Xiaodong Tao

Lang Yuan

Gregory Boverman

Christopher James Sevinsky

Laurence Bigio

Chulmin Joo

David LaVan Henderson

Chun Zhan

Christopher Darby Immer

Elizabeth Lockenberg Dixon

Sam Joseph Camardello

Richard A Hogle

Michael S Lazare

Christina Lowes

Richard R McKay

John David Young

Anthony S Bauco

Bahman Ebrahimi Kashef

Robert Christian Tatar

Cemal Cagatay Bilgin

Methods of additively manufacturing a three-dimensional object include irradiating a first build plane region using a first energy beam, irradiating a second build plane region using a second energy beam, and irradiating an interlace region between the first build plane region and the second build plane region. Irradiating the interlace region comprises directing the first energy beam along a first oscillating path and directing the second energy beam along a second oscillating path intersecting and overlapping with the first oscillating path.

Additive manufacturing methods and systems with two beams traveling along opposing, wobbling paths

A system includes a first group of optic lenses within a focusing unit positioned along the propagation direction of a collimated laser beam, the first group of optic lenses separated by a predetermined fixed distance. The first group of optic lenses in conjunction cause the collimated beam to form as an annular beam as it passes through the first group of optic lenses. An axicon lens located distal from the first group of optic lenses along the propagation direction, the axicon lens operable to bifurcate the annular beam into two deflected collimated beam sections, and the axicon lens having a focus that causes the two deflected collimated beam sections to merge at a distance distal from the axicon lens to create an interference pattern region.

Systems and methods for direct laser melting of metals using non-diffracting laser beams

An additive manufacturing system includes a laser device, a build plate, and a scanning device. The laser device is configured to generate a laser beam with a variable intensity. The build plate is configured to support a powdered build material. The scanning device is configured to selectively direct the laser beam across the powdered build material to generate a melt pool on the build plate. The scanning device is configured to oscillate a spatial position of the laser beam while the laser device simultaneously modulates the intensity of the laser beam to facilitate reducing spatter and to facilitate reducing a temperature of the melt pool to reduce overheating of the melt pool.

Systems and methods for dynamic shaping of laser beam profiles in additive manufacturing

An additive manufacturing system includes a laser device, a build plate, and a scanning device. The laser device is configured to generate a laser beam with a variable intensity. The build plate is configured to support a powdered build material. The scanning device is configured to selectively direct the laser beam across the powdered build material to generate a melt pool on the build plate. The scanning device is configured to oscillate a spatial position of the laser beam while the laser device is configured to simultaneously modulate the intensity of the laser beam to thermally control the melt pool.

Systems and methods for dynamic shaping of laser beam profiles for control of micro-structures in additively manufactured metals

The present approach generally relates to systems and methods for implementing energy modulated tomographic imaging of nanoparticles. In certain embodiments, a first energy is used to activate probe particles labeling an anatomy or tissue of interest. The probe particles, once activated, emit photons at a different rate and/or spectrum in response to an underlying physiological event, such as action potentials propagating in the labeled anatomy or tissue. The emitted photons may then be detected and used to map or image the occurrence of the physiological event.

Energy modulated luminescence tomography

Methods comprising the use of photoactivated chemical bleaching for detecting multiple targets in a biological sample are provided. The methods include the steps of providing a biological sample including multiple targets, binding at least one probe to one or more target present in the sample, and detecting a signal from the probe. The method further includes the steps of contacting the sample comprising the bound probe with an electron transfer reagent, as well as an optional additive which prevents target modification during photoactivated chemical bleaching, and irradiating the sample, thereby initiating a photoreaction that substantially inactivates the probe by photoactivated chemical bleaching. The method further includes the steps of binding at least one probe to one or more target present in the sample, and detecting a signal from the probe. The process of binding, defecting and bleaching may be iteratively repeated.

Photoactivated chemical bleaching of dyes

The present invention provides a microscope slide for analyzing a tissue sample. The slide includes an elongate planar substrate, which comprises a first major surface having a sample-affixing area within which a tissue sample may be affixed; a first positive control sample affixed on the first major surface at a first location adjacent to the sample-affixing area; and a second positive control sample affixed on the first major surface at a second location adjacent to the sample-affixing area. The first and second locations are spaced such that quality of the staining of the first and second positive control samples is indicative of the quality of the staining of the tissue sample. Also provided are a kit containing the microscope slide and a method of making and a method of using the microscope slide.

Microscope slides with quality controls thereon

A method for processing and imaging a first and second plurality of samples, comprising processing at least one sample from the first plurality of samples, imaging the at least one sample from the first plurality of samples, while being capable of simultaneously processing at least one sample from the second plurality of samples; and imaging the at least one processed sample from the second plurality of samples.

Systems and methods for processing and imaging of biological samples

Methods for detecting multiple targets in a biological sample are provided. The methods includes contacting the sample with a first probe; physically binding the first probe to a first target; observing a first signal from the first probe; applying a chemical agent to modify the first signal; contacting the sample with a second probe; physically binding the second probe to a second target; and observing a second signal from the second probe. The methods disclosed herein also provide for multiple iterations of binding, observing, signal modification for deriving information about multiple targets in a single sample. An associated kit and device are also provided.

Sequential analysis of biological samples

Some systems described herein include a frequency dependent phase plate for generating multiple phase-contrast images of a sample, each from a different frequency range of light, each phase-contrast image for frequency range of light formed from light diffracted by the sample interfered with undiffracted light that has a frequency-dependent baseline relative phase shift from the phase plate. In some embodiments, the multiple phase-contrast images may be used to generate a quantitative phase image of a sample. The phase-contrast images or the produced quantitative phase image may have sufficient contrast for label-free auto-segmentation of cell bodies and nuclei.

Quantitative phase microscopy for high-contrast cell imaging using frequency domain phase shift

A screening module configured to screen at least a portion of a biological sample disposed on an analysis surface is provided. The screening module comprises a laser source a scanning unit comprising one or more scanning devices, wherein the scanning devices are configured to rotate in an oscillatory scanning motion about an axis of rotation to scan the analysis surface in at least one direction, wherein the scanning unit is physically coupled to the laser source, and a detection unit comprising one or more detection devices.

Systems and methods for screening of biological samples

Methods and systems for digitally enhancing an initial image of a material to which a plurality of stains were previously applied, that generally comprise: unmixing the image into a plurality of individual reconstructed images, each individual image corresponding to one of the stains; estimating a residual image corresponding to the difference between the original image and the reconstructed images; adjusting one or more components of the individual images; mixing the adjusted components using one or more estimated mixing coefficients; and adding the residual image to the mixed adjusted components to generate an enhanced image.

Methods and systems for digitally enhancing an image of a stained material

A microfluidic flow cell subassembly, which may be assembled into a flow cell having fluidic connections outside of the main substrate, is described for encapsulating a sample to allow for subsequent controlled delivery of reagents to the sample, such as multiplexed in situ biomarker staining and analysis. Methods for fabricating the subassembly and assembled flow cell are also provided. The method includes the steps of adhering a gasket layer to a substrate layer, wherein the gasket layer may contain enclosed features and adhering an adherent layer to the gasket layer. The subassembly may be sealed against a solid support to form a flow cell.

Microfluidic chamber device and fabrication

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Niskayuna, NY, United States of America

Robert John Filkins