Kreatech Biotechnology B.v.

Robert Jochem Heetebrij

Jan Reedijk

Hendrik Jan Houthoff

Herman Hendricus Volkers

Tinka Jelsma

Hendrick Jan Houthoff

Hendrik-Jan Houthoff

Hendrikus Johannes Tanke

Eduard Gerhard Talman

Jacky Theo Maria Veuskens

Robertus Petrus Maria Van Gijlswijk

Anton Klaas Raap

R P M Van Gijlswijk

Jan Hendrick Houthoff

Robertus Petrus Maria Van Gijswijk

The invention relates to a method for differentially labelling one or more entities, together comprising distinct reactive sites. The invention further relates to an entity that has been labelled by a method according to the invention and to a diagnostic kit comprising a labelled entity and to a diagnostic kit to employ a method according to the invention.

Differential labelling method

The present invention provides improved methods for labeling nucleotides. The method of the invention for labeling nucleotides comprises the steps of: reacting a reactive moiety of a linker, which linker is a platinum compound having a stabilizing bridge and two reactive moieties, with an electron donating moiety of a spacer, which spacer comprises a chain having at least four atoms and at least one heteroatom in the chain, which spacer further comprises said electron donating moiety at one end of the chain and a reactive moiety at the other end of the chain; reacting the reactive moiety of said spacer with a label; reacting the other reactive moiety of said linker with a nucleotide. A major advantage of the invention is that all nucleotides can be labeled by the method of the invention, whereas until now the attachment of a label was mostly restricted to one or certain nucleotides.

Methods for labeling nucleotides, labeled nucleotides and useful intermediates

The present invention provides a labeled nucleoside having formula: where X represents an aliphatic diamine, and the labeling moiety comprises a label and a spacer, where the spacer is coupled at one end to a platinum atom and at the other end to the label, which spacer comprises a chain having at least four carbon atoms and at least one oxygen atom in the chain. The invention further provides a method for labeling a nucleoside, kits for labeling a nucleoside, and kits for producing a labeled nucleic acid.

The Invention relates to a method for providing at least one selected sequence in a nucleic acid with interstrand cross-links. The method comprises hybridizing at least one selected single strand sequence with a complementary single strand nucleic acid or a functional analogue thereof. The selected sequence or complementary nucleic acid or both comprise a cross-linking agent. The invention also relates to a method, referred to as COBRA, for the labeling of a set of at least two bio-organic molecules with a set of at least two colors, comprising generating said set of colors through combining ratio labeling with binary labeling.

Applications with and methods for producing selected interstrand cross-links in nucleic acids

Trans-platinum compound, and diagnostic kit

Growing community of inventors

Utrecht, Netherlands

Robert Jochem Heetebrij