Trustees of the Colorado School of Mines

Robert Jinkerson

Matthew C Posewitz

Randor Radakovits

Jonathan E Meuser

Victoria H Work

Methods, processes, and systems for the production of lipids and starch from modified algae are disclosed. In one embodiment, the modified algae over-expresses isoamylase and accumulates much higher amounts of starch than unmodified algae. In some embodiments, the modified algae comprises one or more copies of an isoamylase expression construct. In one embodiment, the modified algae is a sta7mutant with a starchless phenotype that has been complemented with one or more copies of the wild-type genomic STA7 isoamylase gene construct. The complemented, modified algae accumulates much greater amount of starch than an unmodified algae and may be used to produce large amounts of starch and/or lipids.

Modified algae for improved biofuel productivity

The present disclosure is directed to novel polynucleotide sequences for use in. The novel polynucleotide sequences include control sequences and coding sequences. Also disclosed are novel gene expression constructs whereinpromoters/control regions are operatively linked toor non-coding sequences. These novel polynucleotide sequences and expression constructs can be introduced intoand can recombine into thegenome. Expression from these polynucleotide sequences and expression constructs can enhancebiomass and/or lipid biosynthesis. Also disclosed are methods for modifying, for example by stably transformingwith nucleic acid sequences, growing the modified, and obtaining biomass and biofuels from the modified

Growing community of inventors

Golden, CO, United States of America

Robert Jinkerson