Novartis Ag

Robert J Tushinski

Richard J Rigg

Sean Forestell

Yakov Bobrov

A device is provided for use in a centrifuge to separate or isolate material (such as pelleted cells, particulates, aggregates, compounds, blood components, and the like) from the liquid in which they are suspended while limiting or preventing loss of any of the resulting separated or pelleted material during further manipulations. The device can be inserted into a standard centrifugation bucket in a centrifuge and holds a flexible centrifuge bag. The device includes at least one damp assembly, which includes a partition that is maintained in an open position during centrifugation and in a closed position at unit gravity, thus isolating separated or pelleted material at the bottom of the flexible bag in the device after centrifugation and preventing the isolated material from being disturbed during liquid changes and other manipulations. The invention also encompasses a method for isolating material in a centrifuge bag following centrifugation utilizing the flexible bag-holding device described herein, as well as a flexible bag to be used with the device.

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Mountain View, CA, United States of America

Robert J Tushinski