Beckman Instruments, Inc.

Beckman Coulter, Inc.

Robert J Obremski

John Warwick Silzel

Yagang Liu

Charles L Dodson

Tsong-Tseh Tsay

Tung Rung Wang

Christopher W Brown

John W Silzel

Russell A Chipman

Bibijana Cercek

Zhou Shaomin

A microscale binding assay, analyte binding array, and kits are disclosed, which exploit the mass action law to harvest analyte from a liquid sample. This is achieved by fabrication of sorbent zones having up to ten times the binding capacity per unit area generally obtained on polystyrene microtiter plates. The resulting arrays substantially deplete the liquid solution of analyte during incubation. Accordingly, the assays respond to total mass of analyte in the sample, not analyte concentration. This approach, coupled with direct fluorescence detection in the NIR, yields maximal signal intensity and low background for optimal sensitivity.

Detection of very low quantities of analyte bound to a solid phase

A system for simultaneously conducting multiple ligand assays on a sample potentially containing target analytes uses as a detector a waveguide having a planar surface with a plurality of probes of known recognition to the target analytes thereon. The probes are in discrete areas on the waveguide. A sample containing target analyte is treated with a light-responsive compound such that it binds to the target analyte to form a conjugate and the conjugate is applied to the probes on the waveguide. A laser light is passed into the planar surface of the waveguide at a plurality of different locations, by causing relative movement between the waveguide and the laser light, so that evanescent waves radiate from the waveguide. Where conjugate has attached to a probe, there is emission of light different from that emitted by a probe without conjugate attached thereto.

System for simultaneously conducting multiple ligand binding assays

A method and apparatus determines the analyte content of a sample by generating first and second input signals and directing the input signals to the sample. The input signals differ in wavelength by at least 3 nanometers. Due to the interaction between the input signals and the sample, first and second output signals are generated. Each output signal comprises a resonant signal whose peak wavelength is substantially independent of the wavelength of the respective input signal, and a non-resonant output signal whose peak wavelength is dependent upon the wavelength of respective input signal. A detector is used to detect the two output signals, and by distinguishing the resonant output signals from the non-resonant output signals, data about the analyte content of the sample is determined. Principal components regression analysis or multivariate quantitative analysis can be applied to the output signals, for the purpose of distinguishing between the resonant and non-resonant signals. The method and apparatus can also distinguish resonant output signals from each other, and non-resonant output signals from each other.

Signal processing for chemical analysis of samples

A multicomponent quantitative analytical method and apparatus wherein the method includes the steps of and the apparatus is capable of performing steps of obtaining a plurality of calibration spectra, transforming the calibration spectra using a transform with orthogonal basis vectors, obtaining a calibration matrix relating the transform spectra to concentrations of analytes in the calibration samples, obtaining a spectrum for an unknown sample, transforming the unknown sample spectrum, and relating the transformed unknown sample spectrum to the calibration matrix to thereby determine the concentration of analytes in the unknown sample.

Multicomponent quantitative analytical method and apparatus

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Yorba Linda, CA, United States of America

Robert J Obremski