Ionics, Inc.

Robert J Mandle

Edgar Haber

Arthur L Goldstein

Wayne A McRae

A process is disclosed for recovering renatured polypeptides and proteins (collectively 'polypeptides') from solution thereof and/or of their S-sulfonate derivatives (collectively 'solution of polypeptides') in denaturants comprising:

Renaturing reversibly denatured polypeptides and proteins by

The disclosure is directed to an improved apparatus and method for isoelectric focusing whereby the reservoirs and recirculating paths in the apparatus are sealed off from the atmosphere. A liquid is employed which contains buffering compounds capable of maintaining a pH gradient in an electric field. A plurality of permeable diaphragms or membranes are used to separate the flows of liquid while allowing interchange of liquid constituents therebetween. A direct current electrical potential is established transverse to the flow, so as to maintain a gradient of pH steps as between successive channels defined by the membranes, and to separate a mixture of amphoteric substances into said channels.

Isoelectric focusing apparatus

Processes and apparatuses are described for recovering components dispersed in an aqueous solution comprising contacting said solution as a first solution with an electrically conducting barrier, diaphragm or membrane which has a high affinity for at least one of said components whereby a substantial fraction of said component is sorbed by said barrier. The barrier is subsequently contacted with a second aqueous solution and a direct electric current is passed through said barrier and second solution in a direction substantially parallel to the smallest dimension of the barrier thereby facilitating desorption of at least one of the sorbed components from the barrier into the second solution which solution is thereafter removed.

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Lexington, MA, United States of America

Robert J Mandle