Travera, Inc.

Massachusetts Institute of Technology

The Broad Institute, Inc.

Robert J Kimmerling

Paul Blainey

Mark L Stevens

Selim Olcum

Scott R Manalis

Soohong Kim

Shijie Nigel Chou

Nicholas L Calistri

Vivian C Hecht

Yehuda Brody

The invention provides devices and methods for linked multimodal measurements of individual particles using a mass sensor and an additional sensor.

Cellular measurement, calibration, and classification

The present invention includes methods and systems for assessing a cellular response to a treatment modality, such as potential drug candidate, by comparing normalized single-cell measurements of cellular properties without the need of a calibration step.

Treatment response assessment using normalized single cell measurements

Systems and methods for flowing particles, such as biological entities, in a fluidic channel(s) are generally provided. In some cases, the systems described herein are designed such that a single particle may be isolated from a plurality of particles and flowed into a fluidic channel (e.g., a microfluidic channel) and/or collected e.g., on fluidically isolated surfaces. For example, the single particle may be present in a plurality of particles of relatively high density and the single particle is flowed into a fluidic channel, such that it is separated from the plurality of particles. The particles may be spaced within a fluidic channel so that individual particles may be measured/observed over time. In certain embodiments, the particle may be a biological entity. Such article and methods may be useful, for example, for isolating single cells into individual wells of multi-well cell culture dishes (e.g., for single-cell analysis).

Systems, articles, and methods for flowing particles

Hydrodynamic Trap Array. The array includes a serpentine bypassing channel including a plurality of trapping pockets disposed therein, the trapping pockets including a ramp entry portion to decrease flow velocity orthogonal to the trapping pocket to increase trapping efficiency. The relative fluid resistances of the trapping pockets and the serpentine bypassing channel are selected such that a slight majority of the flow is diverted to one of the trapping pockets. A pair of microfluidic bypass channels flank the array of traps allowing independent control of upstream and downstream pressures on each side of the array, thereby decoupling flow magnitude in the bypass channels from flow across the trapping pockets.

Hydrodynamic trap array

Growing community of inventors

Cambridge, MA, United States of America

Robert J Kimmerling