Carl Zeiss Microscopy Gmbh

Ist Austria (institute of Science and Technology Austria)

Robert Hauschild

Helmut Lippert

Matthias Wald

Ralf Wolleschensky

Michael Goelles

Christopher Power

Stefan Wilhelm

Joerg Steinert

Wolfgang Bathe

Michael Gölles

Dietmar Schmidt

Oliver Holub

Gunter Möhler

Matthias Danckwerts

Maria Nemethova

Gunter MÖHLER

Jan Alexander Schwarz

A family of microscopes with illumination systems directing a sheet of light having an approximately planar extension in an illumination axis of an illumination beam path and in a transverse axis orthogonally oriented to the illumination axis. the illumination device, emits illumination light and has two illumination beam paths. A first illumination beam path illuminates the sample region from a first direction on the illumination axis and a second illumination beam path illuminates the sample region from a second opposing direction on the illumination axis. A switching element alternately switches the illumination light between the first illumination beam path and the second illumination beam path, or shifts the light sheet in a direction of the detection axis for adjusting or both.

Microscope with a light sheet

An improved evaluation circuit which allows high sensitivity in an economical manner. For this purpose, a shift register having at least one data input, a clock input, a plurality of register stages and at least one data output is provided, wherein the output of the analog-to-digital converter is connected to the data input of the shift register. With a shift register, fluorescent light and scattered light can be temporally distinguished in an economical manner.

Evaluation circuit for an optoelectronic detector and method for recording fluorescence events

A microscope including an illumination device providing a light sheet illuminating a sample region, said sheet having a planar extension along an illumination axis of an illumination beam path and a transverse axis lying normal to the illumination axis. A detection device detects light emitted from the sample region on a detection axis the illumination axis and detection axis as well as the transverse axis and the detection axis being oriented relative each other at an angle unequal zero. The detection device has a detection lens system arranged in the detection beam path and splitting means for splitting the detection beam path into two beam sub-paths. A dichroic beam splitter in the infinity region of the surface detectors is about 3 mm thick. Wobble plate(s) disposed orthogonal to each other relative to the detection axis arranged in one of the two beam sub-paths so measured values can be automatically superimposed.

Microscope having light sheet illumination of a sample region

A family of microscopes with illumination systems directing a sheet of light having an approximately planar extension in an illumination axis of an illumination beam path and in a transverse axis orthogonally oriented to the illumination axis. The microscopes have detection devices used to detect light that is emitted by a sample region. The detection devices including a detection lens system disposed in the detection beam path and an optical detection element spaced from a front lens of the detection lens system and independently adjustable thereof. The optical detection element continuously varies the size of a detection image field and/or continuously displaces a focal plane of detection in the P-region.

Microscope with a sheet of light

A family of microscopes include an illumination device which produces a planar light sheet along an illumination axis of an illumination beam path and a transverse axis normal to the illumination axis. A detection device detects light emitted from the sample region along an axis of detection of a detection beam path. The illumination and detection axes as well as the transverse axis and the axis of detection being oriented relative each other at an angle unequal to zero. A light sheet generator also produces rotationally symmetrical light and includes structure and control for rapidly scanning the sample region along the transverse axis. The illumination device includes a second light sheet generator having a first astigmatically active optical element with at least one astigmatic lens for producing a static sheet of light. Selection elements used to select either the first or the second light sheet or both together to produce the sheet of light.

Microscope with light sheet illumination

A microscope including an illumination device for a light sheet having an approximately planar extension along an illumination axis of an illumination beam path with a transverse axis to the illumination axis. Light emitted from the sample region on axis of detection the illumination axis and the axis of detection as well as the transverse axis and the axis of detection being oriented relative at an non-zero angle. The illumination device includes structure deflecting light to different beam path and thus produces an additional sheet of light, together illuminating the sample region on common illumination axis, and with switches between beam paths. Detection device has a detection lens system for light from by the sample region. The switches include a rapidly switching element with a time &#x3c;10 ms, a predetermined integration of the surface detector synchronized so the sample region is illuminated twice during integration.

Microscope with illumination switching for capturing sample images during detector integration time

A laser scanning microscope has an illumination beam path and a detection beam path. A beamsplitter is provided which reflects the illumination light in direction of the sample and transmits the detection light in direction of the detection arrangement. An additional beamsplitter is provided for reflecting the illumination light and for transmitting the detection light, this additional beamsplitter being arranged in the illumination beam path downstream of the first beamsplitter in the illumination direction, and this additional beamsplitter substantially transmits the illumination light reflected at the first beamsplitter and the detection light, but acquires a wavelength range substantially different from the first beamsplitter with respect to its reflectivity.

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Vienna, Austria

Robert Hauschild