US Government As Represented by the Secretary of the Army

Robert Haggins, Jr

Timothy Varney

Erik Eaton, Jr

Zachary Murray

An apparatus for isolating corneal endothelial cells (CECs) includes a housing with an interior wall that defines a hollow interior of the housing. At least four fluid channels are formed in the housing with two of the channels terminating in respective openings in an upper portion of the interior wall and two of the channels terminating in respective jets in the upper portion of the interior wall. A convex projection is centrally located on the circular base of the hollow interior and is configured to receive an inverted cornea. A circular insert for the housing has an opening therein in which the convex projection projects when the insert is mated with the lower portion of the interior wall. CECs are harvested from the inverted cornea.

Device and method for isolation of corneal endothelial cells

An apparatus () for isolating corneal endothelial cells () (CECs) includes a base portion () having an interior recessed opening () with a bottom surface (). A convex projection () is centrally located on the bottom surface () and is configured to receive an inverted cornea (). A top portion () is configured to mate with the base portion (). The top portion () includes a fluid chamber () with a lower surface (). The lower surface () has an opening () therein in which the convex projection () projects when the top portion () is mated with the base portion ().

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Belcamp, MD, United States of America

Robert Haggins, Jr