University of California

Other

Robert H Edwards

Christopher J Evans

Duane E Keith, Jr

Daniel L Kaufman

Richard J Reimer

William J Rutter

Erik Jorgensen

Steven L McIntire

Kim Schuske

Robert T Fremeau, Jr

Elizabeth E Bellocchio

Recombinant forms of isolated and purified delta opioid receptors and methods to screen for compounds interactive with delta opioid receptors are described.

DNA molecules encoding opioid receptors and methods of use thereof

Genes encoding opioid receptors (including opioid-like receptor (ORL) proteins) can be retrieved from vertebrate libraries using the murine probe disclosed herein under low-stringency conditions. The DNA sequence shown inor its complement can be used to obtain the human delta, kappa and mu genes as well as the murine mu gene and human ORL-1. The probe provided encodes the murine delta opioid receptor.

Opioid receptors and methods of use

This invention pertains to the identification of a novel class of glutamate transporters. In particular, this invention pertains to the discovery that proteins originally considered to perform an entirely different function (BNPI, DNPI, etc.), in fact, transport glutamate into synaptic vesicles. Designated VGLUT glutamate transporters, the transporters provide good targets with which to screen for modulators of glutamate uptake into synaptic vesicles.

Glutamate transporters

Disclosed are invertebrate and vertebrate synaptic vesicle amino acid transporter compositions which define a novel family of transporter proteins, recombinant vectors and cells comprising nucleic acid sequences encoding vertebrate synaptic vesicle amino acid transporter proteins and antibodies directed against such proteins. Also disclosed are methods for utilizing such compositions in screening assays and treatment regimens.

Vesicular amino acid transporter composition and method

Genes encoding opioid receptors (including opioid-like receptor (ORL) proteins) can be retrieved from vertebrate libraries using the murine probe disclosed herein under low-stringency conditions. The DNA sequence shown in FIG. 5 or its complement can be used to obtain the human delta, kappa and mu genes as well as the murine mu gene and human ORL-1. The probe provided encodes the murine delta opioid receptor.

Methods of screening modulators of opioid receptor activity

Genes encoding opioid receptors can be retrieved from vertebrate libraries using the murine probe disclosed herein under low-stringency conditions. The DNA sequence shown in FIG.,or its complement can be used to obtain the human delta, kappa and mu genes as well as the murine mu gene. The probe provided encodes the murine delta opioid receptor.

Opioid receptor genes

Nucleic acid molecules comprising a nucleotide sequence encoding a mammalian delta opioid receptor are disclosed. The invention provides recombinant materials for the production of mammalian delta opioid receptors.

Nucleic acid encoding delta opioid receptor

Complete cDNA and amino acid sequences are disclosed for rat adrenal-specific and brain-specific transport protein, as well as for human brain-specific transport protein. Methods for obtaining the genes encoding these proteins and for obtaining recombinantly produced protein are described. Antibodies and methods for isolating additional vesicle membrane transport proteins are also described. Methods for using the vesicle membrane transport proteins to identify compounds that selectively inhibit transport of toxic molecules into vesicles, and that prevent inhibition of transport of toxic molecules are also provided. The invention includes methods to treat and diagnose diseases associated with sequestration of toxic molecules in mammalian cells.

Vesicle membrane transport proteins

Star ornament light

A method for producing pure, active, mature recombinant Nerve Growth Factor-beta is disclosed, as is the protein so produced.

Growing community of inventors

Los Angeles, CA, United States of America

Robert H Edwards