Purdue Research Foundation

Amgen Inc.

The Dow Chemical Company

University of Pennsylvania

Finnigan Corporation

Rudue Research Foundation

Mims Technology Development Co.

Indian Institute of Technology Madras

Robert Graham Cooks

Zheng Ouyang

Dalton Snyder

Zane Baird

Alan Keith Jarmusch

Ahmed Mohamed Hamid

Livia Schiavinato Eberlin

Wen-Ping Peng

He Wang

Jason David Harper

Nicholas Alan Charipar

Jiangjiang Liu

Nicholas E Manicke

Qian Yang

Anyin Li

Ziqing Lin

Chien-hsun Chen

Xin Li

Xin Yan

Guangtao Li

Sandilya Venkata Garimella

Jason Duncan

Adam Hollerbach

Michael Stanley Wleklinski

Guangming Huang

Ewa Sokol

Christopher Pulliam

Christina Ramires Ferreira

Ryan M Bain

Tawnya Flick

Pu Wei

Depanjan Sarkar

Michael Pugia

Liang Gao

Allison Lisa Dill

Amar S Basu

Nicolás M Morato

Lucas Szalwinski

Demian R Ifa

Fred Paul Mark Jjunju

Iman Salem Roqan

Justin M Wiseman

Zoltán Takáts

Yafeng Li

Zhenwei Wei

Bogdan Gologan

Stephen Ayrton

David Logsdon

Valentina Pirro

Dylan T Holden

Rahul Narayanan

Soumabha Bag

Ismael Cotte-Rodriguez

David G Mclaren

Brett M Marsh

Kevin Scott Kerian

Joshua Wiley

Qingjie Luo

Zhiping Zhang

James C Tou

William Johnson Chappell

David H Thompson

George C Stafford, Jr

Mark E Bier

James Mitchell Wells

Da Ren

Jonathan W Amy

Robert E Santini

Keyong Hou

Livia Schiavinato

Wei Xu

Zoltan Takáts

Michael P Goodwin

Andre Venter

Thomas Blake

Ethan R Badman

Scott J Bauer

Manish H Soni

Vladimir E Frankevich

Mario Nappi

Jennifer S Brodbelt

Lemoyne B Westover

Pallab Basuri

Zinia Jaman

Tiago Sobreira

Roy Helmy

Ryan Espy

Yangjie Li

Iman S Rogan

Yong Liu

Justin Michael Wiseman

Zoltan Takats

The invention generally relates to mass tag analysis for rare cells and cell free molecules. In certain embodiments, the invention provides an apparatus including an essentially non-absorbent membrane having at least one pore, a microwell operably associated with the essentially non-absorbent membrane, and an electric field generator. The apparatus may be configured such that an electric field produced by the electric field generator operably interacts with a sample in the microwell and expels a droplet of the sample through the at least one pore in the essentially non-absorbent membrane. In certain embodiments, apparatuses of the invention are used for detection, and optionally quantification, of a target analyte from a heterogeneous sample, such as a rare target analyte (e.g., rare cell) from a biological sample.

Mass tag analysis for rare cells and cell free molecules

The invention generally relates to systems and methods for on-line reaction monitoring. In certain embodiments, the invention provides systems that include a reaction vessel having an outlet, a quantitation unit coupled to the outlet and configured to introduce internal standard and solvent into reaction solution flowed from the reaction vessel, one or more ion generating devices that receive flow from the quantitation unit, and a mass spectrometer. In certain embodiments, the invention provides systems for multiple reaction monitoring that include a plurality of reaction vessels, a plurality of ion generating devices, a plurality of channels, each channel coupling a reaction vessel to an ion generating device, an actuator coupled to the plurality of ion generating devices to thereby allow movement of the plurality of ion generating devices, and a mass spectrometer.

Reaction monitoring

The invention generally relates to mass spectrometry via frequency tagging.

Mass spectrometry via frequency tagging

The invention generally relates to methods and devices for synchronization of ion generation with cycling of a discontinuous atmospheric interface. In certain embodiments, the invention provides a system for analyzing a sample that includes a mass spectrometry probe that generates sample ions, a discontinuous atmospheric interface, and a mass analyzer, in which the system is configured such that ion formation is synchronized with cycling of the discontinuous atmospheric interface.

Synchronization of ion generation with cycling of a discontinuous atmospheric interface

The invention generally relates to systems and methods for isolating a target ion in an ion trap. In certain aspects, the invention provides a system that includes a mass spectrometer having an ion trap, and a central processing unit (CPU). The CPU includes storage coupled to the CPU for storing instructions that when executed by the CPU cause the system to apply a dual frequency waveform to the ion trap that ejects non-target ions from the ion trap while retaining a target ion in the ion trap.

Systems and methods for isolating a target ion in an ion trap

The invention generally relates to systems and methods for increasing reaction yield. In certain embodiments, the invention provides systems for increasing a yield of a chemical reaction that include a pneumatic sprayer configured to generate a liquid spray discharge from a solvent. The solvent includes a plurality of molecules, a portion of which react with each other within the liquid spray discharge to form a reaction product. The system also includes a collector positioned to receive the liquid spray discharge including the unreacted molecules and the reaction product. The system also includes a recirculation loop connected from the collector to the pneumatic sprayer in order to allow the unreacted molecules and the reaction product to be recycled through the pneumatic sprayer, thereby allowing a plurality of the unreacted molecules to react with each other as the unreacted molecules cycle again through the system.

Systems and methods for increasing reaction yield

The invention generally relates to conducting reactions in Leidenfrost-levitated droplets.

Conducting reactions in Leidenfrost-levitated droplets

The invention generally relates to systems and methods for synthesizing a reaction product and increasing yield of the same. In certain aspects, the invention provides a multi-mode reaction system in which in a first mode one or more valves of the system are configured to cause reagents to flow from one or more reagent reservoirs of the system through one or more components of the system to generate a reaction product; and in a second mode the one or more valves are configured to change flow within the system such that the one or more reagent reservoirs are isolated from a remainder of the system and the generated reaction product and any remaining reactants are recycled back through the multi-mode reaction system to increase a yield of the reaction product.

Systems and methods for synthesizing a reaction product and increasing yield of the same

The invention generally relates to two-dimensional mass spectrometry using ion micropacket detection. In certain aspects, the invention provides systems including a mass spectrometer having an ion trap and one or more detectors. The system includes a central processing unit (CPU), and storage coupled to the CPU for storing instructions that when executed by the CPU cause the system to: apply one or more scan functions to the ion trap that excite a precursor ion and eject a product ion from the ion trap; and determine a secular frequency of the product ion by detecting micropackets of the product ion as the micropackets are ejected from the ion trap.

Two-dimensional mass spectrometry using ion micropacket detection

The invention generally relates to systems and methods for collision induced dissociation of ions in an ion trap. In certain aspects, the invention provides a system that includes a mass spectrometer having an ion trap, and a central processing unit (CPU). The CPU includes storage coupled to the CPU for storing instructions that when executed by the CPU cause the system to generate one or more signals, and apply the one or more signals to the ion trap in a manner that all ions within the ion trap are fragmented at a same Mathieu q value.

Systems and methods for collision induced dissociation of ions in an ion trap

The invention generally relates to systems and methods for separating ions at about or above atmospheric pressure. In certain embodiments, the invention provides systems that include an ionization source that generates ions and an ion trap. The ion trap is maintained at about or above atmospheric pressure and includes a plurality of electrodes and at least one inlet configured to receive a gas flow and at least one outlet. The system is configured such that a combination of a gas flow and one or more electric fields produced by the electrodes separates the ions based on mass-to-charge ratio and sends the separated ions through the at least one outlet of the ion trap.

Systems and methods for separating ions at about or above atmospheric pressure

The invention generally relates to electrophoretic mass spectrometry probes and systems and methods of uses thereof. In certain aspects, the invention provides a mass spectrometry probe having a hollow body with a distal tip, an electrically conductive hollow conduit, and an electrode. The electrically conductive hollow conduit may be operably coupled to a reservoir and a power source, and the electrically conductive hollow conduit may be configured to transport a liquid sample into the hollow body and polarize the liquid sample as it flows through the electrically conductive hollow conduit and into in the hollow body. The electrode and the electrically conductive hollow conduit are disposed within the hollow body (e.g., at different heights within the hollow body).

Electrophoretic mass spectrometry probes and systems and uses thereof

The invention generally relates to high-throughput label-free enzymatic bioassays using desorption electrospray ionization-mass spectrometry (DESI-MS).

High-throughput label-free enzymatic bioassays using DESI-MS

The invention generally relates to systems and methods for conducting reactions and screening for reaction products.

Systems and methods for conducting reactions and screening for reaction products

The invention generally relates to systems and methods for sample analysis using swabs. In certain aspects, the invention provides systems that include a probe having a conductive proximal portion coupled to a porous material at a distal portion of the probe that is configured to retain a portion of a sample that has contacted the porous material, and a mass spectrometer having an inlet. The system is configured such that the porous material at a distal portion of the probe is aligned over the inlet of the mass spectrometer.

Systems and methods for sample analysis using swabs

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West Lafayette, IN, United States of America

Robert Graham Cooks