New Jersey Institute of Technology

Robert Gang Luo

Kamalesh K Sirkar

Xiao-Ping Dai

Yanke Xu

Yun Kang

An apparatus and method for recovering bioproducts from a feed solution. In one embodiment, the apparatus includes a module housing, a membrane means disposed in the housing for filtering the bioproducts from the feed solution wherein a portion of the membrane means is coated with a polymeric coating, and an adsorbent bed disposed in the housing for retaining the bioproducts which permeate through the membrane, wherein the apparatus is adapted to allow fractionation and purification of the retained bioproducts from the bed by elution.

Method and apparatus for isolation and purification of biomolecules

The present invention relates to methods for depleting endotoxin from biological solutions by exposure of solutions containing endotoxin to an immobilized metal affinity chromatography matrix composed of a metal ion such as iron (III) bounds to a resin, wherein the metal is capable of selectively binding endotoxin in the solution. These methods are useful for purifying, for example, biological products prepared using bacterial cells, or products in which bacterial contamination is problematic. In another embodiment, endotoxin in circulation in a body can be removed by exposing the blood to the aforementioned matrix, whereby endotoxin is removed before the blood is returned to the body.

Methods for removing endotoxins from biological solutions using immobilized metal affinity chromatography

An apparatus and method for recovering bioproducts from a feed solution. In one embodiment, the apparatus includes a module housing, a membrane disposed in the housing for filtering the bioproducts from the feed solution, and an adsorbent bed disposed in the housing for retaining the bioproducts which permeate through the membrane, wherein the apparatus is adapted to allow fractionation and purification of the retained bioproducts from the bed by elution.

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Edison, NJ, United States of America

Robert Gang Luo