Affymetrix, Inc.

Phylos, Inc.

Bristol-myers Squibb Compnay

Labcyte Inc.

Compound Therapeutics, Inc.

Insilixa, Inc.

Cowper Sciences Inc.

Robert G Kuimelis

Glenn H McGall

Martin J Goldberg

Richard W Wagner

Guangyu Xu

Glenn K Fu

Dasa Lipovsek

Pei-Hua Wang

Jing Hu

Richard N Ellson

Joseph D Olechno

Glenn H Mcgall

Zihui Chen

Stephen P A Fodor

Arjang Hassibi

Arun Manickam

Peter Lohse

Rituraj Singh

Gaurav Saini

David Smith

Michael McPherson

Tommy Armsby

Nineveh Parker

Sample container for holding and transferring a liquid sample and method thereof. The sample container includes an inlet configured to allow a liquid sample to enter a sample container, and an outlet configured to allow one or more droplets of the liquid sample to exit the sample container by one or more acoustic ejections respectively. The inlet and the outlet are in different locations.

Sample containers adapted for acoustic ejections and sample preservation and methods thereof

Provided herein are molecules and salts thereof, arrays containing molecules and salts thereof, solid supports containing molecules and salts thereof, kits containing molecules or salts thereof, and methods of determining alignment of photolithographic masks comprising molecules or salts thereof.

Methods and systems for mask alignment in manufacturing process of arrays

The present disclosure provides methods, apparatus and systems for time-gated fluorescent-based detection. Time-based fluorescence analysis can be used in certain biochemical assays by measuring the emitted photon flux from fluorophores after an individual excitation pulse.

Methods and systems for time-gated fluorescent-based detection

Methods are provided for multiplexed amplification of selected targets and analysis of the amplified targets. In preferred aspects the amplification and analysis take place on the same solid support and preferably in a localized area such as a bead or a feature of an array. Targets are circularized by hybridization to probes followed by ligation of the ends of the target to form a closed circle. The targets are then used as template for extension of an array bound probe resulting in extended probes having multiple copies of the target. The extended probes can then be analyzed. The methods may be used for genotyping, sequencing and analysis of copy number.

Amplification and analysis of selected targets on solid supports

Silanation compositions containing a mixture of two or more silanation reagents, where at least one silanation reagent includes a functional group capable of supporting polymer synthesis and at least one silanation reagent includes no functional group capable of supporting polymer synthesis are useful in modulating the active site density and hydrolytic stability of a surface. These compositions are particularly useful in silanating a surface prior to preparation of a polymer array and provide for increased hybridization results.

Silane mixtures

Disclosed herein are proteins that include an immunoglobulin fold and that can be used as scaffolds. Also disclosed herein are nucleic acids encoding such proteins and the use of such proteins in diagnostic methods and in methods for evolving novel compound-binding species and their ligands.

Protein scaffolds for antibody mimics and other binding proteins

Disclosed are compositions and methods for the labeling of two or more targets with different labels. Specifically, disclosed are compositions for biotin and the protection of biotin within multilabel assays which employ the biotin-biotin binding protein binding relationship for each distinct label in relation to targets such as nucleic acids, polypeptides, antibodies or cells. These multilabel assays are enabled through the use of biotin with desthiobiotin, orthogonal protecting schemes for biotin, or a combination of the approaches.

Methods and compositions for selective labeling of different biotinylated  targets within multicolor or multilabel assays

The present invention provides massively parallel oligonucleotide synthesis and purification for applications that utilize large collections of defined high-fidelity oligonucleotides (e.g., from about 10to about 10different sequences, generally between 25-160 bases in length).

Parallel preparation of high fidelity probes in an array format

Methods are presented for generating large sets for polymers. The methods employ high density oligonucleotide array.

Primer array synthesis and validation

Protective groups which may be cleaved with an activatable deprotecting reagents are employed to achieve a highly sensitive, high resolution, combinatorial synthesis of pattern arrays of diverse polymers. In preferred embodiments of the instant invention, the activatable deprotecting reagent is a photoacid generator and the protective groups are DMT for nucleic acids and tBOC for amino acids. This invention has a wide variety of applications and is particularly useful for the solid phase combinatorial synthesis of polymers.

Use of acid scavengers for the synthesis of standard length and long-mer nucleic acid arrays

Growing community of inventors

Palo Alto, CA, United States of America

Robert G Kuimelis