Life Technologies Corporation

Applied Biosystems, LLC

Robert G Eason

Khairuzzaman Bashar Mullah

Handong Li

Zhaochun Ma

Umberto Ulmanella

Vladimir Ivanovich Bashkirov

Bradford J Taft

Maxim G Brevnov

Aldrich Lau

Kevin Hacker

Aldrich N K Lau

Timothy Z Liu

Kristian Michael Scaboo

Vissarion Aivazachvili

Konrad Faulstich

K Bashar Mullah

Methods and kits for preparing nucleic acid fragments from a sample of purified nucleic acid are provided. Alternatively, chromatin or other long polymers can be sheared with similar methods and kits.

Methods and apparatuses for nucleic acid shearing by sonication

Methods for preparation of 2',3&#x2032;-dideoxynucleotides support structures, such as 2&#x2032;,3&#x2032;-dideoxyguanosine, 2&#x2032;,3&#x2032;-dideoxyadenosine, and 3&#x2032;-deoxythymidine support structures are disclosed. Various methods of using such structures are also provided, such as their use for automated DNA synthesis and pyrophosphorolysis activated polymerization.

Synthesis of 2',3&#x2032;-dideoxynucleosides for automated DNA  synthesis and pyrophosphorolysis activated polymerization

Magnetic beads that include polyvalent ligands comprising various carbohydrates are described. Methods for fabricating such magnetic beads are also provided as well as methods of their use to capture and enrich pathogen cell population for subsequent culture, lysis and identification.

Magnetic beads having surface glycoconjugates and use thereof

Synthesis of 2', 3&#x2032;-dideoxynucleosides for automated DNA synthesis and pyrophosphorolysis activated polymerization

System and methods for detecting analytes such as pathogenic cells are described. The methods allow for the direct measurement of analytes such as pathogenic organisms without the need for sample preparation and/or PCR. The devices can be used individually as point-of-use sensors for airborne pathogens and other pathogenic organisms in foods and agriculture products.

Devices and methods for the detection of analytes

Synthesis of 2',3&#x2032;-dideoxynucleosides for automated DNA synthesis and pyrophosphorolysis activated polymerization

Disclosed are tri-nuclear metal complexes and salts thereof, such as tri-nuclear osmium or ruthenium complexes or salts thereof, suitable for use as electrochemical labels. Also disclosed are oligonucleotide probes with an attached electrochemical label, methods of nucleic acid amplification, methods of sequencing, and kits for nucleic acid amplification and sequencing having oligonucleotide probes including an electrochemical label. The electrochemical labels are synthesized from siderophores.

Electrochemical labels derived from siderophores

Growing community of inventors

Los Gatos, CA, United States of America

Robert G Eason