Molecular Devices Corporation

First Medical, Inc.

Metra Biosystems, Inc.

Robert F Zuk

Viola T Kung

John Wallace Parce

Richard D Armenta

Michael P Allen

Vartan E Ghazarossian

Edward L Sheldon

John D Olson

Lawrence M Ensler

Mary J Cerelli

Charles R Burke

Anne R Kopf-Sill

Jonathan Briggs

Yuri Daniloff

Hsin-Shan Julia Ju

Sae Hyun Choo

The present invention encompasses a capture membrane comprising a porous filter membrane having a hapten bound directly or indirectly to the membrane wherein complexes formed by specific binding having an anti-hapten bound to a binding member of the specifically binding complex are removed from a solution by the hapten as the solution passes through the membrane. In the preferred embodiment biotin is the hapten and avidin or streptavidin is the anti-hapten.

Hapten derivatized capture membrane and diagnostic assays using such membrane

Hapten derivatized capture membrane and diagnostic assays using such

The system for transferring plasma to a test substrate comprises an applicator and the test substrate. The applicator includes a blood reservoir and a filter element which separates cellular components from the blood as plasma is passed to the test substrate. The test substrate includes an absorptive element which receives the plasma from the applicator. In one embodiment, measured amounts of plasma are transferred when the absorptive element becomes saturated, thus stopping the flow of plasma. In another embodiment, excess blood and cellular components are removed from the measured plasma by disengaging the applicator from the test substrate. In another embodiment, plasma transferred to the absorptive element is eluted from the absorptive element by subsequent application of an elution medium.

Method for plasma separation and measurement

A fluorescent labelling composition comprises a linear polysaccharide backbone molecule having a plurality of target-binding molecules, such as antibodies or nucleic acids, attached at spaced-apart intervals thereon. Each of the target-binding molecules, in turn, includes a multiplicity of fluorescent dye molecules bound thereto. In this way, fluorescent signal introduced to a single target-site on a solid phase surface may be increased without loss of binding activity.

Fluorescent labelling compositions and methods for their use

A method of assaying bone collagen degradation activity in a human subject. In the method, a human urine sample is reacted with an antibody which (i) is capable of reacting immunospecifically with pyridinium crosslinks selected from the group consisting of native free pyridinoline and native free deoxypyridinoline, and (ii) has a ratio of reactivity toward the selected pyridinium crosslinks and urinary pyridinium peptides larger than 1,000 daltons in molecular weight, of greater than about 5:1. An immunocomplex forms between the antibody reagent and the selected pyridinium crosslinks which are present in the sample, and the amount of immunocomplex is measured. Also disclosed are antibody reagents and kits which can be used in the method.

Method and kit for pyridinium crosslink assay

Compounds and methods for detecting the incorporation of a hapten into a protein or macromolecule are disclosed. The compounds comprise haptens bound directly or indirectly to a chromophoric group and a macromolecule-reactive group, whereby incorporation of the hapten into a protein or other macromolecule may be detected.

Chromophoric reagents for incorporation of biotin or other haptens into

System and method for plasma separation and measurement

A method of assaying a human urine sample, by measuring a concentration of pyridinium crosslinks from which the concentration of total hydrolysed pyridinoline in the sample can be determined, is disclosed. In the method, a urine sample is reacted with an anti-Pyd antibody reagent which preferably has a ratio of reactivity toward native free pyridinoline and urinary pyridinoline peptides larger than 1,000 daltons in molecular weight, of greater than 10:1. By measuring the extent of immunocomplex formed by the reaction, the concentration of total hydrolysed pyridinoline in the sample can be calculated. Also disclosed are an antibody reagent and kit which can be used in the method.

Method and kit for pyridinoline assay

Methods and apparatuses are described for determining the presence of an analyte in a sample suspected of containing the analyte. The method comprises contacting a concentrate of a determinable element capable of being detected by means of a semiconductive electrode with the electrode in the presence of a relatively large volume of an assay medium. The determinable element is present in the concentrate in the amount related to the amount of analyte present in the sample. The volume of the medium in diffusive communication with the concentrate is then reduced and the determinable element is detected by means of the effect that the determinable element has on the semiconductive electrode.

Zero volume electrochemical cell

Compounds and methods for detecting the incorporation of a hapten into a protein or macromolecules are disclosed. The compounds comprise haptens bound directly or indirectly to a chromophoric group and a macromolecules-reactive group, whereby incorporation of the hapten into a protein or other macromolecules may be detected. The disclosure includes a compound of the formula: ##STR1## wherein n.sub.1 is 1, 2, 3, 4, 5, 6 or 7;

An analyzer with an improved rotor structure for analyzing solid-containing and solid free liquids having an improved rotor design that utilizes chambers and channels that operate by centrifugal force and capillary action to separate and meter the liquids for testing purposes.

Analyzer with improved rotor structure

Novel methods and devices are provided for detecting analytes, where the sample is placed upstream from a reagent which is a component of a signal-producing system. The detection zone is provided upstream from the sample, where the amount of reagent which becomes bound at two sites is determined and the two values used for an accurate determination of an analyte.

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Burlingame, CA, United States of America

Robert F Zuk