Nanosphere, Inc.

Nanoink, Inc.

Northwestern University

Amersham Biosciences Ab

Robert Elghanian

Chad A Mirkin

Robert L Letsinger

Robert C Mucic

James J Storhoff

Thomas Andrew Taton

Sylvain Cruchon-Dupeyrat

Hua Zhang

Linette Demers

Nabil A Amro

Sandeep Disawal

David M Kelso

Joseph S Fragala

Viswanadham Garimella

Igor Touzov

Michael R Nelson

Debjyoti Banerjee

Zhi Li

Charles K Brush

Kenneth Luehrsen

Vineet Gupta

So-Jung Park

Sujit Rao Jangam

Abhijit Mazumder

John Edward Bussan

Abhishek K Agarwal

Carl Yamashiro

Chang-Gong Liu

Arkadiy Silbergleyt

Todd Tuggle

Handy Yowanto

Ekaterina Pestova

David R Fermin

David G Wang

Zhijie John Gu

Gang Lu

Yanzheng Xu

Percy Van Crocker

Buena Chui

Krishnamurthy Jayaraman

Gretchen Kiser

Changming Li

Ramesh Ramakrishnan

Provided herein are devices, systems, and methods for specimen preparation by employing a combination of capillary and centrifugal forces. For example, provided herein are devices, systems, and methods that collect a sample by capillary force, separate components of the collected sample by centrifugal force, and isolate one or more of the separated components by further capillary force.

Devices, systems, and methods for specimen preparation using capillary and centrifugal forces

A device for collecting, treating and dispensing a biological sample is described. Use of the device permits integration of sample collection, sample treatment, and sample dispensing.

Methods and devices for sample collection, treatment and dispensing

The invention provides methods of detecting a nucleic acid. The methods comprise contacting the nucleic acid with one or more types of particles having oligonucleotides attached thereto. In one embodiment of the method, the oligonucleotides are attached to nanoparticles and have sequences complementary to portions of the sequence of the nucleic acid. A detectable change (preferably a color change) is brought about as a result of the hybridization of the oligonucleotides on the nanoparticles to the nucleic acid. The invention also provides compositions and kits comprising particles. The invention further provides methods of synthesizing unique nanoparticle-oligonucleotide conjugates, the conjugates produced by the methods, and methods of using the conjugates. In addition, the invention provides nanomaterials and nanostructures comprising nanoparticles and methods of nanofabrication utilizing nanoparticles. Finally, the invention provides a method of separating a selected nucleic acid from other nucleic acids.

Nanoparticles having oligonucleotides attached thereto and uses therefor

A new, low temperature method for directly writing conductive metal traces with micron and sub-micron sized features. In this method, a flat beam is used, such as an AFM cantilever, with or without a tip, to draw traces of metal precursor ink onto a substrate. The dimensions of the metal traces can be directly controlled by the geometry of the cantilever, so that one can controllably deposit traces from 1 micron to over 100 microns wide with microfabricated cantilevers. Cantilevers with sharp tips can be used to further shrink the minimum features sizes to sub-micron scale. The height of the features can be increased by building layers of similar or different material.

Micrometric direct-write methods for patterning conductive material and applications to flat panel display repair

Reagents useful for attaching biomolecules (e.g., proteins, oligonucleotides, and other biomolecules) to a surface, processes of attaching molecules to a surface to form modified surfaces using these reagents, and methods of detecting a target compound using these modified surfaces are disclosed.

Universal phosphoramidite for preparation of modified biomolecules and surfaces

Inkwells adapted for use in direct-write nanolithography and other applications including use of wells, channels, and posts. The wells can possess a geometry which matches the geometry of tips which are dipped into the inkwells. The channels can be open or closed. Hydrophilicity and hydrophobicity can be used to control ink flow. SEM can be used to characterize the inkwells. Ink flow can be monitored with video. Hydrophobic material layers can be used to prevent cross contamination. Microsyringes can be used to fill reservoirs. Satellite reservoirs can be used to prevent bubble formation.

Methods and apparatus for ink delivery to nanolithographic probe systems

Photomask repair and fabrication with use of direct-write nanolithography, including use of scanning probe microscopic tips (e.g., atomic force microscope tips, etc.) for deposition of ink materials including sol-gel inks. Additive methods can be combined with subtractive methods. Holes can be filled with nanostructures. Heights of the nanostructures filling the holes can be controlled without losing control of the lateral dimensions of the nanostructures. Phase shifters on phase shifting masks (PSMs) are additively repaired with selectively deposited sol-gel material that is converted to solid oxide, which has optical transparency and index of refraction adapted for the phase shifters repaired.

Methods for additive repair of phase shift masks by selectively depositing nanometer-scale engineered structures on defective phase shifters

A novel method of transporting ink to a substrate with dip-pen nanolithographic (DPN) stamp tips coated with polymer (e.g., polydimethylsiloxane (PDMS), etc.). This kind of tip adsorbs chemicals ('inks') easily and is used to generate DPN nanopatterns that are imaged with the same tip after a DPN process. This method builds a bridge between micro-contact printing (&#x3bc;CP) and DPN, making it possible for one to easily generate smaller structures of any molecules that have been patterned by the &#x3bc;CP technique. The easy tip-coating and writing process enriches the state-of-the-art DPN technique. The sub-100 nm DPN resolution obtained by using this kind of novel tip is comparable to that with a conventional SiNprobe tip. Importantly, the unique stamp tip was able to transfer solvent (e.g., liquid 'ink') onto a substrate, resulting in fabrication of hollow nanostructures with only one DPN holding/writing step. Inks comprising metals and sol-gel materials are noted, as well as applications in photomask repair, enhancement, and fabrication.

Direct-write nanolithography method of transporting ink with an elastomeric polymer coated nanoscopic tip to form a structure having internal hollows on a substrate

This invention relates to methods and compositions for determining single nucleotide polymorphisms (SNPs) in P450 genes. In preferred embodiments, self extension of interrogation probes is prevented by using novel non self-extension probes and/or methods, thereby improving the specificity and efficiency of P450 SNP detection in target samples with minimal false positive results. The invention thus describes a variety of methods to decrease self-extension of interrogation probes. In addition, this invention provides a unique collection of P450 SNP probes on one assay, primer sequences for specific amplification of each of the seven P450 genes and amplicon control probes to evaluate whether the intended p450 gene targets were amplified successfully. The invention also describes a variety of array platforms for performing the assays of the invention; for example: CodeLink&#x2122;, eSensor&#x2122;, multiplex arrays with cartridges etc., all described herein.

P450 single nucleotide polymorphism biochip analysis

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Skokie, IL, United States of America

Robert Elghanian