Eli Lilly and Company

Robert E Weeks

Kurt E Merkel

Manuel Debono

Charles L Hershberger

Gene M Wild

Herald J Cole

Glycopeptide antibiotic A-4696G is produced by culturing Actinoplanes missouriensis mutant strain ATCC 31681 under submerged aerobic fermentation conditions, and is recovered from the fermentation broth by resin adsorption and chromatographic purification. A-4696G inhibits the growth of bacteria pathogenic to man and animals and also enhances the growth of ruminants by increasing feed utilization.

Antibiotic A-4696 factor G

Antibiotic A-4696 factors B.sub.1, B.sub.2, B.sub.3, C.sub.1a, C.sub.3, and E.sub.1 are isolated from antibiotic A-4696, the latter being produced by Actinoplanes missouriensis strains ATCC 31680, ATCC 31682, and ATCC 31683 under submerged aerobic conditions in a culture medium and isolated from the fermentation broth by absorption on an ion exchange resin and eluted therefrom at p.sup.H 10.5 with sodium hydroxide. The novel factors are separated by thin layer chromatography and high pressure liquid chromatography and have antimicrobial and growth promotant activity.

Biologically pure culture of Actinoplanes missouriensis

Antibiotic A-4696 factors B.sub.1, B.sub.2, B.sub.3, C.sub.1a, C.sub.3

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Indianapolis, IN, United States of America

Robert E Weeks