Scarab Genomics, LLC

Novagen, Inc.

Pel-freez Rabbit Meat, Inc.

Pel-freez

Robert E Novy

Frederick R Blattner

Robert C Mierendorf

David A Frisch

Eric A Steffen

Hyunsic Choi

Charles Landry

Gyorgy Posfai

John Brandon

Terrance M Henker

Gary L Milburn

Michael J Domanico

Scott A Monsma

David O'Reilly

Christopher R Blattner

Jose A Sanchez

Cristopher R Blattner

Beth Hammer

Richard Garber

Keith W Yaeger

Warren Kroeker

Kristin M Kolb

Charles F Landry

Provided herein arehost strains with improved capacity for producing recombinant proteins.

Reduced genomelacking toxin-antitoxin genes

Provided herein are methods for the improved production of periplasmic-targeted recombinant proteins inhost strains. Also provided arehost strains with improved capacity for producing recombinant proteins.

Methods and compositions for improved expression of recombinant proteins

Rediced genome or native K12 strainbacteria comprising expression vectors encoding a recombinant CRM197 protein and their use in the production of CRM 197 is provided. The CRM 197 protein may be fused to a signal sequence that directs the expressed CRM197 protein to the periplasm of thehost.

Enhanced production of recombinant CRM197 in

bacteria comprising genetic modifications to enhance fermentability and production of protein and nucleic acids are provided.

Bacteria with improved metabolic capacity

A triple host vector is described which is capable of expressing an inserted protein coding DNA sequence in any of three distinct host systems. The vector includes three promoters, one each for vertebrate, bacterial and insect host cells, so that the protein coding sequence can be cloned into the vector once and the vector can be used to express protein in all three types of host cells. The arrangement and selection of the promoters contributes to expression characteristics in all three host systems that is comparable to single host expression vectors.

Multiple host expression vector

An antibody raised against an epitope of a peptide having an amino acid sequence selected from a group consisting of Leu Asp Gly Tyr Pro Ser Asp Tyr Asp Pro Phe Val Glu Asn Ala Gln Ile Tyr (SEQ ID NO:1) and Leu Asp Gly Tyr Pro Ser Asp Tyr Asp Pro Phe Glu Glu Asn Ala Gln Ile Tyr (SEQ ID NO:2) is advantageously used in an indirect immunoassay to detect the presence of active Human Herpesvirus-6 in a patient sample.

Antibody to carboxy-terminus of human herpesvirus 6 immediate early

Recombinant rabbit tissue factor is cloned and produced in a bacterial host. This protein, which is relatively insoluble and has several disulfide bonds, requires special modifications in order to express and be purified at commercial levels. By expressing the tissue factor as a fusion protein with a bacterial enzyme, thioredoxin, solubility is increased. Use of a thioredoxin reductase deficient host aids in proper tertiary structure for biological activity.

Recombinant rabbit tissue factor

A vector for the direct cloning of the products of PCR protocol incorporates single nucleotide overhangs at one or both ends of a linearized DNA segment. The single nucleotide overhangs are uracil or inosine residues, as desired, to facilitate cloning of the desired PCR products.

Direct cloning of DNA fragments

An antibody raised against an epitope of a peptide having an amino acid sequence selected from a group consisting of LDGYPSDYDPFVENAQIY (SEQ ID NO:1) and LDGYPSDYDPFEENAQIY (SEQ ID NO:2) is advantageously used in an indirect immunoassay to detect the presence of active Human Herpesvirus-6 in a patient sample.

Human herpesvirus 6 detection assay

A method for determining the nucleic acid sequence that encodes ligand binding domain on any protein encoded by a known gene includes the steps of non-specifically cleaving the coding region of the known gene, size-selecting the cleavage products, cloning the size-selected gene products into an expression vector active in a heterologous host, using a novel cloning strategy expressing the peptides produced by the clones and screening for clones that produce peptides that interact with an antibody or ligand of interest.

Growing community of inventors

Verona, WI, United States of America

Robert E Novy