Thermo Fisher Scientific Oy

Cohesive Technologies, Inc.

Robert Dewitte

Joseph L Herman

Vesa Nuotio

John Edward Brann, Iii

Terry N Olney

Juhani Siidorov

Jukka Saukkonen

Dayana Argoti

Jeffrey A Zonderman

Raimo Salminen

Joseph M Senteno

Jarmo Vehkomaki

Bill Ostman

John Edward Iii Brann

Systems, apparatuses, kits, and methods for purification and analysis of analytes having a broad range of hydrophobicities by liquid chromatography-mass spectrometry (LC-MS). Using one set of liquid chromatography columns, one set of mobile phase buffers, and, optionally, a single ionization method (e.g., electrospray ionization), a wide range of analytes can be purified and analyzed on a liquid chromatography-mass spectrometry (LC-MS) system. LC-MS purification and analysis of analytes having a broad range of partition coefficients is accomplished by selecting LC run parameters and MS system parameters that are particular to different classes of analytes without having to make column or buffer changes or any other hardware configuration changes to the LC-MS system. The methods, systems, and kits described herein provide for substantially increased speed/throughput and ease of use for a wide range analytes with essentially no compromise in specificity for individual analytes relative to previously described methods.

LC-MS configuration for purification and detection of analytes having a broad range of hydrophobicities

A method of multiplexing the operation of a sample preparation and analysis system is disclosed. The system includes a sample preparation system capable of preparing a first sample in accordance with a first assay that is selected from a database containing a plurality of unique assays and preparing a second sample in accordance with a second, different assay. The method includes separately transporting the first and second prepared samples to an analysis station having first and second separation channels and an analyzer comprising a mass spectrometer. The method further includes simultaneously analyzing the first prepared sample with the analyzer in accordance with the first selected assay and separating the second prepared sample with the second separation channel in accordance with the second selected assay.

Automated system for sample preparation and analysis

LC-MS configuration for purification and detection of analytes having a broad range of hydrophobicites

A sample preparation and analysis system. The system includes a sample preparation system and a sample analysis system. The sample preparation system prepares samples in accordance with an assay that is selected from a database containing a plurality of unique assays. The sample analysis system includes an analyzer that is dynamically reconfigurable based on the selected assay so as to analyze the prepared sample in accordance with that selected assay. A data communication link communicates data from the sample preparation system to the sample analysis system to reconfigure the analyzer in accordance with the selected assay.

A sample preparation and analysis system. The systemincludes a sample preparation systemand a sample analysis system. The sample preparation systemprepares samples in accordance with an assay that is selected from a database containing a plurality of unique assays. The sample analysis systemincludes an analyzerthat is dynamically reconfigurable based on the selected assay so as to analyze the prepared sample in accordance with that selected assay. A data communication linkcommunicates data from the sample preparation systemto the sample analysis systemto reconfigure the analyzerin accordance with the selected assay.

A sample preparation and analysis system. The system includes a housing with a sample preparation station and a sample analysis station positioned within the housing. The sample analysis station is spaced away from the sample preparation station. A transport assembly is configured to move at least one sample within the housing and between the sample preparation station and the sample analysis station.

System layout for an automated system for sample preparation and analysis

A sample preparation and analysis system (). The system () includes a housing () with a sample preparation station () and a sample analysis station () positioned within the housing (). The sample analysis station () is spaced away from the sample preparation station (). A transport assembly () is configured to move at least one sample within the housing () and between the sample preparation station () and the sample analysis station ().

Growing community of inventors

Burlington, Canada

Robert Dewitte