Natural Environment Research Council

Oxford Brookes University

Roussel Uclaf

Robert David Possee

Linda Anne King

Patricia Jane Cayley

Miguel Lopez Ferber

Lorna Mary Dyet Stewart

Described herein are baculovirus vectors, host cells containing the baculovirus vectors, methods of using the baculovirus vectors for cloning genes, and kits containing the baculovirus vectors.

Baculovirus expression system

The application relates for method for cloning the gene comprising the steps of: 1. Providing a replication-deficient baculovirus vector, 2. Providing a rescue vector comprising (a) nucleic acid sequence which is capable of restoring replication in the replication-deficient baculovirus vector and (b) at least one gene to be cloned; 3. Causing the replication-deficient baculovirus vector and rescue vector to recombine to produce a replication-enabled baculovirus vector comprising the at least one gene to be cloned; and 4. Growing the replication-enabled baculovirus vector within a suitable invertebrate cell, such as an insect cell. Preferably the baculovirus vector is based upon AcMNPV. Also disclosed are replication-deficient baculovirus vectors, rescue vectors, cells containing such vectors and kits comprising such vectors.

A recombinant baculovirus which, in insect cells infected therewith, expresses a foreign protein comprising an insecticidal toxin, or a functional derivative thereof, provided with a signal peptide such that the toxin or derivative thereof is secreted from the insect cells. This recombinant baculovirus can be used in insect control.

Growing community of inventors

Witney, United Kingdom

Robert David Possee