University of Colorado

Robert D Niederriter

Juliet T Gopinath

Diego Restrepo

Emily A Gibson

Stephanie A Meyer

Mark E Siemens

Brendan M Heffernan

Sub-diffraction limited fluorescent images using a fiber-based stimulated emission depletion (STED) microscope are reported. Both excitation and depletion beams are transported through polarization-maintaining fiber and a lateral resolution of 100 nm has been achieved.

Methods and systems for stimulated emission depletion microscopy

A compact diode laser achieves high-power, short duration output pulses by separating the lasing action from the pulse-generating mechanism. A diode seed source is configured for gain-switching via a variable RF source. A time lens element includes an intensity modulation device, a phase modulation device, and a pulse compressor. The intensity modulation device carves shorter pulses from the long gain-switched seed pulses, the phase modulation device adds chirp, and the pulse compressor compensates for the chirp while producing high-power short-duration output pulses.

Compact diode laser source

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Los Angeles, CA, United States of America

Robert D Niederriter