University of Georgia Research Foundation, Inc.

Synageva Biopharma Corporation

Avigenics, Inc.

University of Iowa Research Foundation

Synageva Biopharm Corp.

Robert D Ivarie

Alex J Harvey

Julie A Morris

Guodong Liu

Jeffrey C Rapp

Tracy M Andacht

Markley C Leavitt

Jeff Rapp

Arthur Karnuah

Gordon Speksnijder

The invention includes isolating pharmaceutical proteins from avian hard shell eggs containing pharmaceutical proteins wherein the pharmaceutical proteins are exogenous to the egg.

Method of making protein in an egg of a transgenic chicken

This invention provides for proteins which are expressed in the avian oviduct, packaged into eggs laid by the avian, then isolated.

Production of exogenous proteins in egg whites of transgenic chickens

This invention provides vectors and methods for the stable introduction of exogenous nucleic acid sequences into the genome of a bird and for expressing said exogenous sequences to alter the phenotype of the bird or to produce desired proteins. In particular, transgenic chickens are produced which express exogenous sequences in their oviducts. Eggs which contain exogenous proteins are also produced.

Transgenic chickens producing exogenous protein in eggs

The invention provides for antibodies produced in transgenic birds as chickens.

Antibodies produced in a transgenic avian egg white

This invention provides for proteins which are expressed in the avian oviduct, packaged into eggs laid by the avian and removed from the eggs.

Method of producing an exogenous protein in the egg of a chicken

The invention relates to G-CSF molecules having certain O-linked oligosaccharide structures.

Glycosylated human G-CSF

The invention relates to interferon-&#x3b1; molecules having certain O-linked oligosaccharide structures.

Glycosylated interferon alpha

The present invention provides isolated and recombinant avian nucleic acid molecules comprising at least one avian MAR and an avian nucleic acid sequence encoding an ovalbumin transcriptional regulatory region. The isolated nucleic acid of the present invention is useful for reducing chromosomal positional effects upon the transcription of a transgene operably linked to the ovalbumin transcriptional regulatory region and transfected into a recipient avian cell The recombinant nucleic acid molecules of the present invention may further comprise a polyadenylation signal sequence or an avian 3' domain, and optionally, an internal ribosome entry site for expression of an operably linked heterologous nucleic acid insert in a transfected avian cell.

Chicken ovalbumin nucleotide sequence

This invention provides vectors and methods for the stable introduction of exogenous nucleic acid sequences into the genome of avians in order to express the exogenous sequences to alter the phenotype of the avians or to produce desired proteins. In particular, transgenic avians are produced which express exogenous sequences in their oviducts and which deposit exogenous proteins into their eggs. Avian eggs that contain exogenous proteins are encompassed by this invention. The instant invention further provides novel forms of interferon and erythropoietin which are efficiently expressed in the oviduct of transgenic avians and deposited into avian eggs.

Avians expressing heterologous protein

This invention provides eggs which contain exogenous proteins. The invention further provides transgenic chickens which express exogenous sequences in their oviducts, and vectors and methods for the stable introduction of exogenous nucleic acid sequences into the genome of a bird for expressing said exogenous sequences to alter the phenotype of the bird or to produce desired proteins.

Transgenic chickens that lay eggs containing exogenous proteins

Granulocyte colony stimulating factor obtained from eggs laid by transgenic avians having newly described G-CSF glycosylation patterns.

Glycosylated G-CSF obtained from a transgenic chicken

This invention provides for human interferon-&#x3b1; which includes novel glycosylation structures.

Glycosylated interferon &#x3b1;

This invention includes compositions comprising glycosylated human interferon-&#x3b1; derived from a chicken.

Glycosylated interferon alpha obtained from a transgenic chicken

Poultry Derived Glycosylated Interferon Alpha 2b

The present invention relates to isolated or non-natural nucleic acids encoding at least a portion of an avianprotein or a variant thereof. Nucleic acids comprising all or part of the-encoding region and/or an untranslated region of acDNA are used as probes for hybridizing and detecting nucleic acids encoding all or part of aprotein. Recombinant cells, tissues and animals containing recombinant nucleic acids including expression vectors and encoding, antibodies to theproteins, assays utilizing thepolypeptide are within the scope of the present invention. Recombinant nucleic acid molecules may contain transcription regulatory sequences, a sequence complementary to a mRNA sequence encoding a-related polypeptide and transcriptional control sequences functional in a recipient cell. Oligopeptides having amino acid sequences derived from the avianprotein may be used to induce the formation of polyclonal or monoclonal antibodies that specifically bind to the chickenprotein.

Growing community of inventors

Watkinsville, GA, United States of America

Robert D Ivarie