Synthetic Genomics, Inc.

Exxonmobil Research and Engineering Company

Verenium Corporation

Viridos, Inc.

Pfizer Canada Inc.

Robert Christopher Brown

Rekha Seshadri

Toby Howard Richardson

Leah Soriaga

Jennifer Coppersmith

Ariel S Schwartz

Prachee Prakash

Mark Joseph Burk

Dan Eric Robertson

Stephen K Picataggio

Nelson Robert Barton

Kurt Iver Kamrud

David E Lam

Xuqiu Tan

Geoff Hazlewood

Kevin T Watts

Joel Kreps

Giselle G Janssen

Kevin V Martin

Jessica Roxane Kristof

Gena Roy

Amit Vasavada

Svetlana Gramatikova

Stuart A Underwood

Blake G Sturgis

Charles Isaac

Wilhelmus P Van Hoek

Adrian Badillo

Roderick James Fielding

Jincai Li

Peter Luginbuhl

Helge Zieler

John Verruto

Abraham Antonio Anderson

Nicholas Bauman

Erik Holtzapple

Paul Budworth

Soyan Leung Lieberman

Weidong Xu

Shaun Bailey

Jay McCarren

Srividya Akella

Stanley Bower

You Chen

Carlos Chavez-Torres

Thomas E Wall

Jonathan E Meuser

Genevieve Hansen

Scott Kroken

Gordana Djordjevic

Leslie Hickle

Daniel Yee

Jennie Lau Kit

Anna E Romano

Douglas Gillette Smith

The present invention relates to the culture and manipulation of microorganisms for biotech applications, and is based on the discovery and characterization of spliced leader sequences identified in transcripts fromspecies. In particular, the invention provides nucleic acid compositions comprising a SL sequence operably linked to a protein-encoding gene. Further provided are compositions and methods for enhanced gene expression in recombinant microorganisms as well as methods for identification and/or isolation of nucleic acid molecules tagged with a spliced leader sequence.

spliced leader sequences and methods of use thereof

The present invention provides compositions and methods for the production and delivery of recombinant double-stranded RNA molecules (dsRNA) encoding heterologous proteins, which can be useful for various therapeutic purposes as well as for the production of desired proteins. The compositions contain engineered double-stranded RNA particles (dsRPs) that can contain a double-stranded RNA molecule that can be a genome or portion of a genome, which can be enclosed in a capsid or coat protein. The dsRNA molecule also comprises an RNA sub-sequence that encodes a heterologous protein. The dsRPs can be derived from wild-type viral organisms. The delivery of the dsRPs (or DNA or RNA molecules) of the invention to an organism provides for therapeutic benefits as well as for the production of desired proteins.

Recombinant RNA particles and methods of producing proteins

The present invention provides compositions and methods for the production and delivery of recombinant double-stranded RNA molecules (dsRNA) targeting pathogen sequences, which can be useful as an RNA vaccine. The compositions contain engineered double-stranded RNA particles (dsRPs) that can contain a double-stranded RNA molecule that can be a genome or portion of a genome, which can be enclosed in a capsid or coat protein. The dsRNA molecule also comprises an RNA sub-sequence that binds to a target sequence of a pathogenic organism. The dsRPs can be derived from wild-type viral organisms. The delivery of the dsRPs of the invention to an organism provides a protection to the organism from the pathogen.

Recombinant RNA particles and methods of use

Mutant photosynthetic microorganisms having reduced chlorophyll and increased photosynthetic efficiency are provided. The mutants have a locked in high light-acclimated phenotype, in which many of the photosynthetic parameters characteristic of high light acclimated wild type cells are found in the LIHLA mutants when acclimated to low light, such as reduced chlorophyll, reduced NPQ, higher qP, higher E, higher Pper unit chlorophyll with little to no reduction in Pper cell, and higher rates of electron transport through photosystem II over a wide range of light intensities. Provided herein are constructs for attenuating or disrupting genes are provided for generating mutants having the LIHLA phenotype. Also provided are methods of culturing LIHLA mutants for the production of biomass or other products.

Algal mutants having a locked-in high light acclimated phenotype

Nannochloropsis spliced leader sequences and uses therefor

Provided herein are methods for identifying centromeres and centromeres identified by such methods. Centromeres of organisms such as algae, fungi, and protists can be used, for example, for constructing artificial chromosomes and cells containing such artificial chromosomes.

Identification of centromere sequences and uses therefor

The invention relates to methods for producing lipids such as fatty acid products in recombinant host cells engineered to express a non-native gene encoding a dehydrogenase. The recombinant microorganisms are able to proliferate at a higher rate as compared with microorganisms that do not express a non-native dehydrogenase gene, and cultures of microorganisms that are engineered for lipid production and that express a non-native dehydrogenase produce more lipid than cultures of control microorganisms that do not include a non-native dehydrogenase gene.

Cell systems and methods for improving fatty acid synthesis by expression of dehydrogenases

The invention provides transgenic photosynthetic microorganisms that include non-native genes encoding polypeptides having lipolytic activity for production of free fatty acids and fatty acid derivatives, and methods of producing free fatty acids and fatty acid derivatives using the transgenic microorganisms disclosed herein. The invention also provides transgenic microorganisms that include non-native genes encoding polypeptides having lipolytic activity, and novel genes encoding polypeptides demonstrating lipolytic activity.

Production of fatty acids and fatty acid derivatives by recombinant microorganisms expressing polypeptides having lipolytic activity

The present invention provides for the improved production of fatty acid products in a recombinant microorganism or host cell engineered to express a non-native gene encoding a thioesterase and a non-native gene encoding a lysophosphatidic acid acyltransferase (LPAAT), in which the LPAAT is overexpressed in the recombinant microorganism compared to an otherwise identical microorganism that does not include the non-native LPAAT gene. The invention also provides methods of producing fatty acid products using such recombinant microorganisms.

Recombinant microorganisms comprising thioesterase and lysophosphatidic acid acyltransferase genes for fatty acid production

This invention describes genes, metabolic pathways, microbial strains and methods to produce methylbutanol and other compounds of interest from renewable feedstocks.

Methylbutanol as an advanced biofuel

The invention relates to acyl-CoA-independent methods of producing fatty alcohols in recombinant host cells engineered to express an alcohol-forming acyl-ACP reductase. The recombinant host cells may be photosynthetic microorganisms, such as cyanobacteria. Isolated nucleic acid molecules, vectors, and recombinant host cells expressing an alcohol-forming acyl-ACP reductase, and systems for producing fatty alcohols via an acyl-CoA-independent pathway, are also provided. Also provided are microorganisms engineered for the secretion of fatty acids and fatty acid derivatives, including fatty alcohols, and methods of producing fatty acid derivatives using such engineering microorganisms.

Production and secretion of fatty acids and fatty acid derivatives

The described invention provides genetically engineered microorganisms, including photosynthetic microorganisms, expressing 4-hydroxybenzoyl-CoA thioesterases and methods of using the genetically engineered microorganisms for producing free fatty acids and/or fatty acid derivatives.

Genetically engineered microorganisms comprising 4-hydroxybenzoyl-coa thioesterases and methods of using the same for producing free fatty acids and fatty acid derivatives

The present invention relates to compositions for inducing immune responses, including an antigen and a promiscuous T-cell epitope. Also provided are methods of inducing immune responses in hosts, comprising administering compositions comprising antigens and promiscuous T-cell epitopes to the host.

Vaccines

This invention describes genes, metabolic pathways, microbial strains and methods to produce methyl butanol and other compounds of interest from renewable feedstocks.

The invention provides novel polypeptides having phospholipase activity, including, e.g., phospholipase A, B, C and D activity, patatin activity, phosphatidic acid phosphatases (PAP)) and/or lipid acyl hydrolase (LAH) activity, nucleic acids encoding them and antibodies that bind to them. Industrial methods, e.g., oil degumming, and products comprising use of these phospholipases are also provided.

Phospholipases, nucleic acids encoding them and methods for making and using them

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San Diego, CA, United States of America

Robert Christopher Brown