Stratos Genomics, Inc.

Roche Sequencing Solutions, Inc.

Roche Diagnostics Gmbh

F. Hoffmann-la Roche Ag

Robert Busam

Mark Stamatios Kokoris

Marc Prindle

Michael Kovarik

Jack Chase

Miranda Lahman

Melud Nabavi

Craig Ostrander

Samantha Vellucci

Taylor Lehmann

Salka Keller

Greg Thiessen

Megan Murt

Methods and compositions for the selection of nucleic acid processing and other enzymes, and more specifically for the selection of DNA polymerases and other enzymes with desired properties employing the directed evolution of enzymes by plasmid tagging in droplets.

Directed evolution of enzymes by plasmid tagging in droplets

Recombinant DPO4-type DNA polymerase variants with amino acid substitutions that confer modified properties upon the polymerase for improved single molecule sequencing applications are provided. Such properties may include enhanced binding and incorporation of bulky nucleotide analog substrates into daughter strands and the like. Also provided are compositions comprising such DPO4 variants and nucleotide analogs, as well as nucleic acids which encode the polymerases with the aforementioned phenotypes.

DPO4 polymerase variants

Recombinant DP04-type DNA polymerase variants with amino acid substitutions that confer modified properties upon the polymerase for improved single molecule sequencing applications are provided. Such properties may include enhanced binding and incorporation of bulky nucleotide analog substrates into daughter strands and the like. Also provided are compositions comprising such DP04 variants and nucleotide analogs, as well as nucleic acids which encode the polymerases with the aforementioned phenotypes.

DP04 polymerase variants

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Seattle, WA, United States of America

Robert Busam