Bio-rad Laboratories, Inc.

Robert Bruce Wallace

Cynthia K French

Jeffrey Cossman

Ching-I Patsy Lin

Luis A Ugozzoli

Franklin R Witney

Hector Juarez-Salinas

Antonio Arevalo Reyes

The present invention relates to methods for lyophilizing eukaryotic cells and isolating intact nucleic acids from such cells, and related kits for using the same.

Method and formulation for lyophilizing cultured human cells to preserve RNA and DNA contained in cells for use in molecular biology experiments

The extensive synthesis (&ldquo;amplification&rdquo;) of a nucleic acid sequence of interest is attained through a linked series of multi-cycle primer extension reactions (LLA). The primers used in each of the primer extension reactions of the process contain non-replicable and/or cleavable elements that halt nucleic acid synthesis and thereby prevent the synthesized molecules from serving as templates in subsequent cycles. Synthesized molecules accumulate during primer extension in a mathematically linear fashion, thereby rendering the process relatively insensitive to contaminating nucleic acids. Multiple primer sets are employed, and simultaneously included in the reaction mixture, thereby ensuring the accumulation of a large number of copies of the nucleic acid sequence of interest. The invention also provides for the detection of an amplified nucleic acid sequence of interest, as well as reagent kits for carrying out the reaction.

Linked linear amplification of nucleic acids

Method and formulation for lyophilizing cultured human cells to preserve

The extensive synthesis ('amplification') of a nucleic acid sequence of interest is attained through a linked series of multi-cycle primer extension reactions (LLA). The primers used in each of the primer extension reactions of the process contain non-replicable elements that halt nucleic acid synthesis and thereby prevent the synthesized molecules from serving as templates in subsequent cycles. Synthesized molecules accumulate during primer extension in a mathematically linear fashion, thereby rendering the process relatively insensitive to contaminating nucleic acids. Multiple primer sets are employed, thereby ensuring the accumulation of a large number of copies of the nucleic acid sequence of interest. The invention also provides for the detection of an amplified nucleic acid sequence of interest, as well as reagent kits for carrying out the reaction.

This invention provides methods and compounds relating to the use of deoxyribose nicotinamide adenine dinucleotide (dNAD.sup.+) analogues in NAD.sup.+ -dependent ligation reactions. The compounds and methods may be used in NAD.sup.+ -dependent ligation reactions generally, and to increase the specificity of NAD.sup.+ -dependent ligation reactions.

Use of deoxyribose nicotinamide adenine dinucleotide to enhance the

This invention relates to a method of cloning DNA produced by primer extension including PCR amplified, reverse transcriptase-generated or primer extended synthetic DNA. Specifically, it relates to a method in which alkane diol residue containing oligonucleotide primers are incorporated into DNA by primer extension followed by direct cloning of the target DNA. Following transformation, the host excises the alkane diol residue with its endogenous DNA repair machinery.

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Greenbrae, CA, United States of America

Robert Bruce Wallace